Molecular biomarkers used to detect cellular/genetic damage in tissue-engineered skin

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dc.authorscopusid7006776689
dc.authorscopusid7402080469
dc.authorscopusid7201365954
dc.authorscopusid7004108963
dc.authorscopusid7003895386
dc.authorscopusid7003834140
dc.authorscopusid6602782865
dc.contributor.authorO'Connell C.
dc.contributor.authorBarker P.E.
dc.contributor.authorMarino M.
dc.contributor.authorMcAndrew P.
dc.contributor.authorAtha D.H.
dc.contributor.authorJaruga P.
dc.contributor.authorBirincioglu M.
dc.date.accessioned2024-08-04T19:59:14Z
dc.date.available2024-08-04T19:59:14Z
dc.date.issued2004
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIn this study, tissue-engineered skin (TestSkin II) was obtained, separated into its two cellular layers (epidermis and dermis) and DNA was extracted. The first biomarker tested consisted of screening for DNA point mutations in exons 5 and 6 of the TP53 gene, the most commonly mutated gene in skin cancer. To ensure the accuracy of the results, two measurement technologies that incorporate internal calibration standards were used. It was shown that tissue-engineered skin did not contain mutations in this gene at the level of sensitivity of SSCP-capillary electrophoresis and Denaturing High Performance Liquid Chromatography. Results were compared to control cells (neonatal fibroblasts and neonatal keratinocytes) and fibroblasts that were obtained from a 55-year-old and 96-year-old human donor. The second set of biomarkers tested looked at the loss of the Y-chromosome. Using Fluorescent In Situ Hybridization technology, no detectable loss of Y-chromosome was found in the tissue-engineered skin and neonatal control cells. Y-chromosome loss was found in the fibroblasts from the 96-year-old donor. Biomarkers such as TP53 mutations and chromosome loss can provide the basis for an international reference standard of cellular biomarkers that can aid in the development and safety of tissue engineered medical products.en_US
dc.identifier.doi10.1520/stp11650s
dc.identifier.endpage253en_US
dc.identifier.issn0066-0558
dc.identifier.issue1452en_US
dc.identifier.scopus2-s2.0-3242735796en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage246en_US
dc.identifier.urihttps://doi.org/10.1520/stp11650s
dc.identifier.urihttps://hdl.handle.net/11616/90487
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherASTM Internationalen_US
dc.relation.ispartofASTM Special Technical Publicationen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiomarkersen_US
dc.subjectCE-SSCPen_US
dc.subjectDHPLCen_US
dc.subjectTP53en_US
dc.subjectY-chromosomeen_US
dc.titleMolecular biomarkers used to detect cellular/genetic damage in tissue-engineered skinen_US
dc.typeConference Objecten_US

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