Electrochemical poly(1,3-phenylenediamine) synthesis as enzyme immobilization media
Küçük Resim Yok
Tarih
1998
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
John Wiley & Sons Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Electrochemical polymerization of the 1,3-phenylenediamine in the presence of glucose oxidase with KCl aqueous electrolyte at a potential of 0.800 V versus Ag-AgCl produces adherent poly(1,3-phenylenediamine) containing enzyme (glucose oxidase) film on a platinum electrode. Polymeric sensor prepared in this one-step procedure can be used to determine hydrogen peroxide formed as the result of the enzymatic reaction between glucose and glucose oxidase in the presence of O-2. The amperometric responses of the resultant enzyme electrode to glucose were rapid, reaching steady-state values within 4-5 s, and there was a linear relationship between glucose concentration and obtained current up to 6 mM. Polymeric sensor was stable for more 3 months. The glucose selectivity of enzyme electrode was determined in the presence of some interfering substances, such as lactose, sucrose, urea, uric acid, paracetamol, and ascorbic acid. Also, the effects of buffer concentration, storage conditions, and temperature on the steady-state amperometric responses were studied. Moreover, the Arrhenius activation energy for the enzymatic reaction was calculated. (C) 1998 John Wiley & Sons, Inc.
Açıklama
Anahtar Kelimeler
poly(1,3-phenylenediamine), glucose, glucose oxidase, enzyme electrode, amperometric biosensor
Kaynak
Journal of Applied Polymer Science
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
68
Sayı
1
