Periodontal hastalığın patogenezinde mitokondri-endoplazmik retikulum kontakt proteinlerinin incelenmesi
Küçük Resim Yok
Tarih
2025
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Mitokondri ile endoplazmik retikulum arasındaki kontakt (MERK) kalsiyum sinyalizasyonu ve apoptozun düzenlenmesi gibi birçok önemli olayda görev alır. Bu çalışmanın amacı MERK proteinleri VAPB, PTPIP51, IP3R, GRP75 ve VDAC'ın farklı periodontal duruma sahip bireylerde DOS, tükürük ve dişeti düzeylerini araştırmaktır. Aynı zamanda ROS, Ca ve CASP3 düzeylerini de inceleyerek MERK proteinlerin apoptoz, kalsiyum metabolizması ve oksidatif stres ile ilişkisini de araştırmaktır. Materyal ve Metot: Sistemik açıdan sağlıklı 48 birey periodontal durumlarına göre (periodontal sağlıklı (n=16), gingivitis (n=16) ve periodontitis (n=16)) 3 gruba ayrılmıştır. MERK ile ilişkili yapılardan VAPB, PTPIP51, IP3R, GRP75 ve VDAC ile inflamasyon ile ilişkili yapılar CASP3, kalsiyum ve ROS'un tükürük ve DOS düzeyleri ELISA ile incelenmiştir. Gen ekspresyon analizleri için ise 60 adet farklı katılımcı eşit sayıda ve aynı periodontal hastalık gruplarına ayrılmış ve gen ekspresyon düzeyleri dişeti örneklerinde RT-qPCR ile incelenmiştir. Bulgular: Periodontitisli hastalarda VAPB, GRP75, VDAC, CASP3 ve ROS DOS toplam miktarı artmışken PTPIP51 toplam miktarı azalmış olarak bulundu (p<0.05). VAPB, PTPIP51, IP3R, GRP75, VDAC, CASP3, Ca ve ROS DOS konsantrasyonlarının ise periodontitisli hastalarda azaldığı saptandı (p<0.05). Dişetinde ise perioodontitisin varlığının PTPIP51, IP3R, GRP75, CASP3, ROS ve ATP2B1 gen ekspresyon seviyelerini yukarı yönlü regüle ettiği, VAPB ve VDAC gen ekspresyon seviyelerini ise aşağı yönlü regüle ettiği saptandı (p<0.05). CASP3, Ca ve ROS ile PTPIP51 arasında negatif ve GRP75 ile de pozitif korelasyon gözlendi (p<0.05). Sonuç: MERK proteinleri VAPB, GRP75, VDAC ve PTPIP51 periodontal hastalığın patogenezinde rol oynayabilir. Periodontal hastalığın patogenezinde apoptoz, oksidatif stres ve kalsiyum mekanizması ile PTPIP51 ve GRP75 proteinleri ilişkili olabilir. Anahtar Kelimeler: MERK, Periodontitis, Gingivitis, Mitokondri, ER
Objective: Mitochondria–endoplasmic reticulum contact sites (MERCs) are involved in essential cellular processes such as calcium signaling and apoptosis regulation. This study aimed to investigate the levels of MERC-related proteins (VAPB, PTPIP51, IP3R, GRP75, and VDAC) in saliva, gingival crevicular fluid (GCF), and gingival tissue of individuals with varying periodontal conditions. Additionally, it evaluated levels of reactive oxygen species (ROS), calcium, and caspase-3 (CASP3) to explore the relationship between MERC proteins and oxidative stress, calcium metabolism, and apoptosis. Materials and Methods: Forty-eight systemically healthy participants were divided into three groups: periodontally healthy (n=16), gingivitis (n=16), and periodontitis (n=16). Protein levels in saliva and GCF were measured using ELISA. Gingival tissue samples were obtained from 60 individuals across the same groups for gene expression analysis using RT-qPCR. Results: In periodontitis patients, total levels of VAPB, GRP75, VDAC, CASP3, and ROS in GCF were elevated, whereas PTPIP51 was decreased (p<0.05). However, the concentrations of all measured markers in GCF were significantly reduced (p<0.05). In gingival tissue, gene expressions of PTPIP51, IP3R, GRP75, CASP3, ROS, and ATP2B1 were upregulated, while VAPB and VDAC were downregulated (p<0.05). GCF CASP3, calcium, and ROS levels were negatively correlated with PTPIP51 but positively correlated with GRP75 (p<0.05). Conclusion: MERC-related proteins VAPB, GRP75, VDAC, and PTPIP51 may play a role in the pathogenesis of periodontititis. PTPIP51 and GRP75 may be associated with apoptosis, oxidative stress, and calcium mechanisms in the progression of periodontal disease. Keywords: MERC, Periodontitis, Gingivitis, Mitochondria, ER
Objective: Mitochondria–endoplasmic reticulum contact sites (MERCs) are involved in essential cellular processes such as calcium signaling and apoptosis regulation. This study aimed to investigate the levels of MERC-related proteins (VAPB, PTPIP51, IP3R, GRP75, and VDAC) in saliva, gingival crevicular fluid (GCF), and gingival tissue of individuals with varying periodontal conditions. Additionally, it evaluated levels of reactive oxygen species (ROS), calcium, and caspase-3 (CASP3) to explore the relationship between MERC proteins and oxidative stress, calcium metabolism, and apoptosis. Materials and Methods: Forty-eight systemically healthy participants were divided into three groups: periodontally healthy (n=16), gingivitis (n=16), and periodontitis (n=16). Protein levels in saliva and GCF were measured using ELISA. Gingival tissue samples were obtained from 60 individuals across the same groups for gene expression analysis using RT-qPCR. Results: In periodontitis patients, total levels of VAPB, GRP75, VDAC, CASP3, and ROS in GCF were elevated, whereas PTPIP51 was decreased (p<0.05). However, the concentrations of all measured markers in GCF were significantly reduced (p<0.05). In gingival tissue, gene expressions of PTPIP51, IP3R, GRP75, CASP3, ROS, and ATP2B1 were upregulated, while VAPB and VDAC were downregulated (p<0.05). GCF CASP3, calcium, and ROS levels were negatively correlated with PTPIP51 but positively correlated with GRP75 (p<0.05). Conclusion: MERC-related proteins VAPB, GRP75, VDAC, and PTPIP51 may play a role in the pathogenesis of periodontititis. PTPIP51 and GRP75 may be associated with apoptosis, oxidative stress, and calcium mechanisms in the progression of periodontal disease. Keywords: MERC, Periodontitis, Gingivitis, Mitochondria, ER
Açıklama
Anahtar Kelimeler
Diş Hekimliği, Dentistry
