İdrar Örneklerinden İzole Edilen Gram Negatif Bakterilerin Değişik Antibiyotiklere Duyarlılığı
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Dosyalar
Tarih
2002
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi Tıp Fakültesi Dergisi
Erişim Hakkı
Attribution 3.0 United States
Özet
Üriner sistem infeksiyonları her yıl milyonlarca insanı etkileyen ciddi bir sağlık problemidir. Bununla birlikte bu
infeksiyonların büyük bir kısmından sorumlu olan Escherichia coli ve Enterobacteriaeceae üyelerinde antibiyotik
direncinin hızla gelişmesi tedavide büyük sorun oluşturmaktadır.
Bu çalışmanın amacı, idrar örneklerinden izole edilen Gram negatif bakterilerin 12 farklı antibiyotiğe
(ampisilin/sulbaktam, amoksisilin/klavulanik asit, sefalotin, sefoksitin, sefoperazon, gentamisin, amikasin,
tobramisin, trimetoprim/sulfametoksazol, siprofloksasin, imipenem) karşı duyarlılıklarını saptamak olmuştur.
İzole edilen bakterilerin identifikasyonu api 20E (Biomerieux) ve beraberinde konvansiyonel yöntemlerle
gerçekleştirilmiştir. Antibiyotik duyarlılığı Kirby-Bauer disk difüzyon yöntemiyle araştırılmıştır.
İzolatların 42’si (%46.2) E.coli, 18’i (%19.8) Klebsiella pneumoniae, 13’ü (%14.2) Enterobacter cloacae, 11’i (%12.1)
Proteus vulgaris ve 7’si (%7.7) Pseudomonas aeruginosa olarak tanımlanmıştır.
Elde edilen sonuçlara göre incelenen suşlarda amikasin ve imipenem direnci tespit edilmemiştir. Gentamisin ve
tobramisine yüksek düzeyde duyarlılık (%57.0-100) bulunmuştur. Tüm suşlarda ampisilin, ampisilin/sulbaktam ve
amoksisilin/klavulanik asite yüksek düzeyde direnç (%69.0-100) saptanırken trimetoprim/sulfametoksazol
(%38.9-100) ve siprofloksasine (%11.9-100) direnç oranlarında dikkat çekici artış gözlenmiştir. Sefalosporinlerden
sefalotine yüksek düzeyde direnç (%92.8-100) saptanırken, en düşük direnç sefoperazona (%16.7-57.1)
bulunmuştur.
Urinary system infection is a serious health problem that effects millions of people each year. Along with this, the rapid development of antibiotic resistance observed in the Enterobacteriaceae and Escherichia coli species which are responsible for the majority of these infections constitutes a serious problem in its treatment. Thus the objective of this study is to identify the susceptibility of the Gram-negative bacteria isolated from urine samples to 12 different antibiotics (ampicillin, ampicillin/sulbactam, amoxicillin/clavulanic acid, sefalotin, cefoxitin, cefoperazone, gentamicin, amikacin, tobramycin, trimethoprim/sulfamethoxazole, ciprofloxacin and imipenem). In identification of the isolated bacteria api 20E (BioMerieux) has been used together with the known conventional methods. Antibiotic susceptibility is investigated by Kirby-Bauer disc diffusion method. Totally 91 isolated bacteria were examined. These are identified as follows: 42 as Escherichia coli (462%); 18 as Klebsiella pneumoniae (19.8%); 13 as Enterobacter cloacae (14.2%); 11 as Proteus vulgaris (12.1%) and 7 as Pseudomonas aeruginosa (7.7%). During our studies none of the examinated strains showed resistance to amikacin and imipenem, but high level of susceptibility to gentamicin and tobramycin (57.0-100%) was observed. Although high resistance to ampicillin, ampicillin/sulbactam and amoxicillin/clavulanic acid (69.0-100%) was identified in all the strains, the high increase in the resistance to trimethoprim-sulfamethoxazole (38.9-100%) and ciprofloxacin (11.9-100%) was far more considerable. Among the cephalosporins resistance to cefalotin was very high (92.8-100%) while the minimum resistance was identified to cefoperazone (16.7-57.1%).
Urinary system infection is a serious health problem that effects millions of people each year. Along with this, the rapid development of antibiotic resistance observed in the Enterobacteriaceae and Escherichia coli species which are responsible for the majority of these infections constitutes a serious problem in its treatment. Thus the objective of this study is to identify the susceptibility of the Gram-negative bacteria isolated from urine samples to 12 different antibiotics (ampicillin, ampicillin/sulbactam, amoxicillin/clavulanic acid, sefalotin, cefoxitin, cefoperazone, gentamicin, amikacin, tobramycin, trimethoprim/sulfamethoxazole, ciprofloxacin and imipenem). In identification of the isolated bacteria api 20E (BioMerieux) has been used together with the known conventional methods. Antibiotic susceptibility is investigated by Kirby-Bauer disc diffusion method. Totally 91 isolated bacteria were examined. These are identified as follows: 42 as Escherichia coli (462%); 18 as Klebsiella pneumoniae (19.8%); 13 as Enterobacter cloacae (14.2%); 11 as Proteus vulgaris (12.1%) and 7 as Pseudomonas aeruginosa (7.7%). During our studies none of the examinated strains showed resistance to amikacin and imipenem, but high level of susceptibility to gentamicin and tobramycin (57.0-100%) was observed. Although high resistance to ampicillin, ampicillin/sulbactam and amoxicillin/clavulanic acid (69.0-100%) was identified in all the strains, the high increase in the resistance to trimethoprim-sulfamethoxazole (38.9-100%) and ciprofloxacin (11.9-100%) was far more considerable. Among the cephalosporins resistance to cefalotin was very high (92.8-100%) while the minimum resistance was identified to cefoperazone (16.7-57.1%).
Açıklama
İnönü Üniversitesi Tıp Fakültesi Dergisi
9(3) 185-189 (2002)
Anahtar Kelimeler
Üriner Sistem İnfeksiyonları, Gram-Negatif Bakteri, Antimikrobiyal Duyarlılık, Urinary System Infection, Gram-Negative Bacteria, Antimicrobial Susceptibility
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Türkmen, Lale ;İnönü Üniversitesi Tıp Fakültesi Dergisi 9(3) 185-189 (2002)
