Ameliorative Effects of Larazotide Acetate on Intestinal Permeability and Bacterial Translocation in Acute Pancreatitis Model in Rats

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dc.authoridSatilmis, Basri/0000-0002-2538-5774
dc.authoridİnceoğlu, Feyza/0000-0003-1453-0937
dc.authoridKarahan, Doğu/0000-0002-5387-2000
dc.authoridOzyalin, Fatma/0000-0001-6486-6389
dc.authorwosidSatilmis, Basri/JBR-9078-2023
dc.authorwosidİnceoğlu, Feyza/GVK-2847-2022
dc.authorwosidKarahan, Doğu/HJI-2769-2023
dc.contributor.authorKarahan, Dogu
dc.contributor.authorHarputluoglu, Muhsin Murat Muhip
dc.contributor.authorGul, Mehmet
dc.contributor.authorGunduz, Ayten
dc.contributor.authorOzyalin, Fatma
dc.contributor.authorInceoglu, Feyza
dc.contributor.authorTikici, Deniz
dc.date.accessioned2024-08-04T20:55:08Z
dc.date.available2024-08-04T20:55:08Z
dc.date.issued2024
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground Intestinal barrier dysfunction in acute pancreatitis (AP) may progress to systemic inflammatory response syndrome (SIRS) and multi-organ failures by causing bacterial translocation. Larazotide acetate (LA) is a molecule that acts as a tight junction (TJ) regulator by blocking zonulin (Zo) receptors in the intestine. Aims In our study, we aimed to investigate the effects of LA on intestinal barrier dysfunction and bacterial translocation in the AP model in rats. Methods Thirty-two male Sprague-Dawley rats were divided into 4 groups; control, larazotide (LAR), AP, and AP + LAR. The AP model was created by administering 250 mg/100 g bm L-Arginine intraperitoneally 2 times with an hour interval. AP + LAR group received prophylactic 0.01 mg/mL LA orally for 7 days before the first dose of L-Arginine. For intestinal permeability analysis, fluorescein isothiocyanate-dextran (FITC-Dextran) was applied to rats by gavage. The positivity of any of the liver, small intestine mesentery, and spleen cultures were defined as bacterial translocation. Histopathologically damage and zonulin immunoreactivity in the intestine were investigated. Results Compared to the control group, the intestinal damage scores, anti-Zo-1 immunoreactivity H-Score, serum FITC-Dextran levels and bacterial translocation frequency (100% versus 0%) in the AP group were significantly higher (all p < 0.01). Intestinal damage scores, anti-Zo-1 immunoreactivity H-score, serum FITC-Dextran levels, and bacterial translocation frequency (50% versus 100%) were significantly lower in the AP + LAR group compared to the AP group (all p < 0.01). Conclusions Our findings show that LA reduces the increased intestinal permeability and intestinal damage by its effect on Zo in the AP model in rats, and decreases the frequency of bacterial translocation as a result of these positive effects.en_US
dc.description.sponsorshipInn niversitesien_US
dc.description.sponsorshipNo Statement Availableen_US
dc.identifier.doi10.1007/s10620-024-08326-8
dc.identifier.endpage1252en_US
dc.identifier.issn0163-2116
dc.identifier.issn1573-2568
dc.identifier.issue4en_US
dc.identifier.pmid38441784en_US
dc.identifier.scopus2-s2.0-85186612197en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1242en_US
dc.identifier.urihttps://doi.org/10.1007/s10620-024-08326-8
dc.identifier.urihttps://hdl.handle.net/11616/101859
dc.identifier.volume69en_US
dc.identifier.wosWOS:001176416500001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofDigestive Diseases and Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLarazotide acetateen_US
dc.subjectAcute pancreatitisen_US
dc.subjectIntestinal permeabilityen_US
dc.subjectZonulinen_US
dc.subjectBacterial translocationen_US
dc.titleAmeliorative Effects of Larazotide Acetate on Intestinal Permeability and Bacterial Translocation in Acute Pancreatitis Model in Ratsen_US
dc.typeArticleen_US

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