Evidence for the presence of a second electron donor for cytoplasmic thioredoxins in the yeast s cerevisiae

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dc.authorid110769en_US
dc.authorid24898en_US
dc.authorid21863en_US
dc.contributor.authorKoç, Ahmet
dc.contributor.authorKarakaya, Hüseyin Çağlar
dc.contributor.authorÜnlü, Ercan Selçuk
dc.date.accessioned2017-06-19T06:45:26Z
dc.date.available2017-06-19T06:45:26Z
dc.date.issued2006
dc.departmentİnönü Üniversitesien_US
dc.descriptionT Journal Biol.en_US
dc.description.abstractIn yeast, the cytoplasmic thioredoxin system is composed of NADPH, thioredoxin reductase-1 (TRR1) and 2 thioredoxin genes (TRX1, TRX2). In this study, using yeast knockout mutants for TRR1, TRX1 and TRX2 genes, the role of the thioredoxin system in methionine sulfoxide reduction was investigated. Cells lacking both TRX1 and TRX2 genes simultaneously were not able to reduce methionine sulfoxides to methionine; however, mutants missing the TRR1 gene were able to reduce methionine sulfoxides to methionine, which showed that electrons could be transferred from NADPH to thioredoxins in the absence of TRR1. Similar results were observed for 3-phosphoadenosine 5-phosphosulfate reduction in the inorganic sulfate assimilation pathway. Results from both assays suggested that yeast cells have additional cytoplasmic thioredoxin reductase activity that could compensate for methionine sulfoxide reduction and sulfate assimilation in the absence of TRR1. This report also constitutes the first evidence that thioredoxins are the in vivo electron donors for methionine sulfoxide reductases in yeast.en_US
dc.identifier.citationKOÇ, A., ÜNLÜ, E. S., & KARAKAYA, H. Ç. (2006). Evidence for the Presence of a Second Electron Donor for Cytoplasmic Thioredoxins in the Yeast S cerevisiae . T Journal Biol, (133), 133–0.en_US
dc.identifier.endpage0en_US
dc.identifier.issue0en_US
dc.identifier.startpage133en_US
dc.identifier.urihttp://journals.tubitak.gov.tr/biology/issues/biy-06-30-3/biy-30-3-3-0508-2.pdf
dc.identifier.urihttps://hdl.handle.net/11616/7102
dc.identifier.volume133en_US
dc.language.isoenen_US
dc.publisherT Journal Biolen_US
dc.relation.ispartofT Journal Biolen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectThioredoxin reductaseen_US
dc.subjectThioredoxinen_US
dc.subjectMethionineen_US
dc.subjectMethionine sulfoxideen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.titleEvidence for the presence of a second electron donor for cytoplasmic thioredoxins in the yeast s cerevisiaeen_US
dc.typeArticleen_US

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