Investigation of Carbapenemase-Producing Pseudomonas aeruginosa at Secondary Care Hospital in Bolu, Turkey

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dc.contributor.authorCekin, Zuhal Kalayci
dc.contributor.authorTanriverdi, Elif Seren
dc.contributor.authorOtlu, Baris
dc.date.accessioned2026-04-04T13:33:31Z
dc.date.available2026-04-04T13:33:31Z
dc.date.issued2024
dc.departmentİnönü Üniversitesi
dc.description.abstractThe global increase in carbapenem resistance poses a significant public health threat due to the potential emergence of multidrug-resistant pathogens and limited treatment options. To learn more about this issue and offer potential solutions, we conducted a study of carbapenem-resistant Pseudomonas aeruginosa (CRPA) infections in a secondary care hospital setting. The study utilized the carbapenem inactivation method (CIM), a leading phenotypic analysis, to determine carbapenemase activity in 63 CRPA isolates. Additionally, polymerase chain reaction (PCR) analysis was conducted to test for the presence of carbapenemase genes associated with the production or expression of various carbapenemase enzymes, including blaKPC, blaNDM, blaVIM, blaOXA-48, blaIMP, and blaGES. Arbitrary primed PCR (AP-PCR) was performed to assess the clonal relationship between different isolates. The isolates were also classified as either health care-associated infections or community-acquired infections, and their clonal relationship and gene positivity were evaluated. A total of 63 CRPA samples underwent evaluation, with 14 isolates determined to be carbapenemase producers via CIM tests. PCR assays revealed that 14 isolates carried carbapenemase genes, with 9 carrying blaNDM, 2 carrying blaGES, 2 carrying blaVIM, and 1 carrying blaIMP. CRPA exhibited a 22% prevalence of carbapenemase genes, of which 64% were attributed to the NDM gene responsible for multidrug resistance. AP-PCR revealed high clonal diversity among the isolates. Molecular epidemiological evaluation also showed no dominant outbreak strain among PA isolates. This study presents significant data on the prevalence and distribution of carbapenemase-producing CRPA strains isolated from secondary health care facilities. Typically, the literature focuses on resistance rates in tertiary care public hospitals. These findings may aid in understanding resistance and its mechanisms, as well as in developing effective treatment strategies and infection control measures.
dc.identifier.doi10.1089/mdr.2024.0067
dc.identifier.endpage457
dc.identifier.issn1076-6294
dc.identifier.issn1931-8448
dc.identifier.issue11
dc.identifier.orcid0000-0002-0449-0356
dc.identifier.orcid0000-0001-5293-2940
dc.identifier.pmid39535927
dc.identifier.scopus2-s2.0-85209408859
dc.identifier.scopusqualityQ2
dc.identifier.startpage450
dc.identifier.urihttps://doi.org/10.1089/mdr.2024.0067
dc.identifier.urihttps://hdl.handle.net/11616/109203
dc.identifier.volume30
dc.identifier.wosWOS:001353302800003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMary Ann Liebert, Inc
dc.relation.ispartofMicrobial Drug Resistance
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250329
dc.subjectpseudomonas aeruginosa
dc.subjectcarbapenem resistance
dc.subjectNDM
dc.subjectGES
dc.titleInvestigation of Carbapenemase-Producing Pseudomonas aeruginosa at Secondary Care Hospital in Bolu, Turkey
dc.typeArticle

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