Quantitative determination of some phenolics in Origanum laevigatum Boiss. extracts via validated LC-MS/MS method and antioxidant activity
Küçük Resim Yok
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Acg Publications
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, quantities of some phenolics; caffeic acid, (E)-ferulic acid, rosmarinic acid, chlorogenic acid, fumaric acid, gallic acid, pyrogallol and vanillin in the directly methanol (M1), chloroform (C), acetone (Ac) and methanol (M2) extratcs obtained from Origanum laevigatum Boiss. from Turkey were investigated via liquid chromatography and tandem mass spectrometry (LC-MS/MS). Curcumin was used as an internal standard. Caffeic acid, chlorogenic acid, rosmarinic acid and gallic acid were determined as the most abundant phenolic acids in the studied extracts using LC-MS/MS. In LC-MS/MS measurements relative standard deviations (RSD %) for caffeic acid, (E)-ferulic acid, rosmarinic acid, chlorogenic acid, fumaric acid, gallic acid, pyrogallol and vanillin were found to be 8.04, 5.21, 5.45, 3.73, 5.44, 4.85, 5.47 and 6.57 % respectively. For the investigated analytes, the correlation coefficient was found in the range of 0.9803 to 0.9981. Furthermore, antioxidant properties of the extracts were determined based on 2,2-diphenyl-1-picrylhydrazyl (DPPH), beta-carotene linoleic acid and cupric (Cu2+) ion reducing power assay (CUPRAC). Acetone (Ac) and methanol (M2) extracts showed high activity in all test assays due to their high concentration of rosmarinic acid, caffeic acid and gallic acid in the extracts. (C) 2018 ACG Publications. All rights reserved.
Açıklama
Anahtar Kelimeler
Origanum, phenolics, LC-MS/MS, validation, antioxidant activity, rosmarinic acid
Kaynak
Journal of Chemical Metrology
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
12
Sayı
2
