Comparison of Conventional Methods, Automated Systems, and DNA Sequence Analysis Methods in the Identification of Corynebacterium afermentans and Corynebacterium mucifaciens Bacteria Isolated from Blood and Catheter Culture Samples

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dc.authoridparlak, mehmet/0000-0001-9542-5121
dc.authoridPARLAK, Mehmet/0000-0001-6030-2244
dc.authorwosidparlak, mehmet/ABB-8651-2020
dc.authorwosidPARLAK, Mehmet/A-8579-2018
dc.contributor.authorOlmez, Serpil
dc.contributor.authorTuncer, Ozlem
dc.contributor.authorParlak, Mehmet
dc.contributor.authorBicakcigil, Asiye
dc.contributor.authorGursoy, Nafia Canan
dc.contributor.authorOtlu, Baris
dc.contributor.authorGuducuoglu, Huseyin
dc.date.accessioned2024-08-04T20:50:25Z
dc.date.available2024-08-04T20:50:25Z
dc.date.issued2021
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe aim of this study is to compare different methods due to the difficulties in identifying coryneform bacteria to species level and to determine antibiotic resistance profiles. Isolates identified as Turicella otitidis (n:45) by VITEK 2 Compact and Corynebacterium mucifaciens (n:1) by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), isolated from blood and catheter cultures between 2015 and 2017 were included in the study. For identification of the isolates, conventional tests and 16S rDNA sequence analysis were performed. Antibiotic susceptibilities of the isolates were determined by Etest. The isolates identified as T. otitidis with VITEK 2 Compact could not be identified by MALDI-TOF MS and described as C. mucifaciens/Corynebacterium afermentans spp. by 16S rDNA sequence analysis. One isolate identified as C. mucifaciens by MALDI-TOF MS could not be identified with VITEK 2 Compact and described as C. mucifaciens by 16S rDNA sequence analysis and conventional methods. All isolates (n:45) described as C. mucifaciens/C. afermentans spp. by 16S rDNA sequence analysis were identified as C. afermentans subsp. afermentans with conventional methods. All 45 isolates identified as C. afermentans subsp. afermentans were resistant to penicillin, erythromycin, and clindamycin and were susceptible to vancomycin and daptomycin, whereas 31 (69%) were resistant to trimethoprim-sulfamethoxazole (TMP-SXT). The isolate identified as C. mucifaciens was susceptible to penicillin, vancomycin, daptomycin, and TMP-SXT; it was resistant to erythromycin and clindamycin. In this study, we reported 45 C. afermentans isolates misidentified as T. otitidis in routine laboratory processes. To our knowledge, this is the first study to include the highest number of C. afermentans blood isolates.en_US
dc.description.sponsorshipResearch Fund of the Yuzuncu Yil University Scientific Research Project [TTU-2017-5729]en_US
dc.description.sponsorshipThis study was supported by the Research Fund of the Yuzuncu Yil University Scientific Research Project as a project numbered TTU-2017-5729.en_US
dc.identifier.doi10.1089/mdr.2020.0039
dc.identifier.endpage923en_US
dc.identifier.issn1076-6294
dc.identifier.issn1931-8448
dc.identifier.issue7en_US
dc.identifier.pmid33411575en_US
dc.identifier.scopus2-s2.0-85110732030en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage919en_US
dc.identifier.urihttps://doi.org/10.1089/mdr.2020.0039
dc.identifier.urihttps://hdl.handle.net/11616/100054
dc.identifier.volume27en_US
dc.identifier.wosWOS:000607140100001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofMicrobial Drug Resistanceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCorynebacterium afermentansen_US
dc.subjectC. mucifaciensen_US
dc.subjectidentificationen_US
dc.subjectantibiotic susceptibility testen_US
dc.titleComparison of Conventional Methods, Automated Systems, and DNA Sequence Analysis Methods in the Identification of Corynebacterium afermentans and Corynebacterium mucifaciens Bacteria Isolated from Blood and Catheter Culture Samplesen_US
dc.typeArticleen_US

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