Cell growth and oxygen uptake of Escherichia coli and Pseudomonas aeruginosa are differently effected by the genetically engineered Vitreoscilla hemoglobin gene

Basit Öğe Kaydı
dc.authoridGeckil, Hikmet/0000-0003-0070-0691
dc.authorwosidStark, Benjamin C/E-6095-2010
dc.authorwosidGeckil, Hikmet/F-7647-2012
dc.contributor.authorGeckil, H
dc.contributor.authorStark, BC
dc.contributor.authorWebster, DA
dc.date.accessioned2024-08-04T20:12:19Z
dc.date.available2024-08-04T20:12:19Z
dc.date.issued2001
dc.departmentİnönü Üniversitesien_US
dc.description.abstractVitreoscilla hemoglobin is a good oxygen trapping agent and its presence in genetically engineered Escherichia coli helps this bacterium to grow better. Here, the potential use of this hemoglobin, for improving the growth and the oxygen transfer properties of Pseudomonas aeruginosa as well as Escherichia coli, was investigated. To stably maintain it in both bacteria, a broad-host range cosmid vector (pHG1), containing the entire coding sequence for Vitreoscilla hemoglobin gene and its native promoter on a 2.3 kb fragment, was constructed. Though at different levels, both bacteria produced hemoglobin and while the oxygen uptake rates of vgb-bearing strains were 2-3-fold greater than that of non-vgb-bearing strains in both bacteria, the growth advantage afforded by the presence of Vitreoscilla hemoglobin was somewhat varied. As an alternative to the traditional method of the improvement of oxygen transfer properties of the environment in which cells are grown, the genetic manipulation applied here improved the oxygen utilization properties of cells themselves. (C) 2001 Published by Elsevier Science B.V.en_US
dc.identifier.doi10.1016/S0168-1656(00)00384-9
dc.identifier.endpage66en_US
dc.identifier.issn0168-1656
dc.identifier.issn1873-4863
dc.identifier.issue1en_US
dc.identifier.pmid11164963en_US
dc.identifier.scopus2-s2.0-0035936617en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage57en_US
dc.identifier.urihttps://doi.org/10.1016/S0168-1656(00)00384-9
dc.identifier.urihttps://hdl.handle.net/11616/93356
dc.identifier.volume85en_US
dc.identifier.wosWOS:000166884300008en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal of Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectVitreoscilla hemoglobinen_US
dc.subjectbacterial hemoglobinen_US
dc.subjectoxygen uptakeen_US
dc.subjectgenetic engineeringen_US
dc.subjectPseudomonas aeruginosaen_US
dc.titleCell growth and oxygen uptake of Escherichia coli and Pseudomonas aeruginosa are differently effected by the genetically engineered Vitreoscilla hemoglobin geneen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu