Correlation of spermiogram profiles with DNA damage in sperm cells of infertile men: a comet assay study

Basit Öğe Kaydı
dc.authoridSandal, Suleyman/0000-0002-8916-3329
dc.authoridYilmaz, Bayram/0000-0002-2674-6535;
dc.authorwosidSandal, Suleyman/AAA-6388-2021
dc.authorwosidYilmaz, Bayram/IXD-3395-2023
dc.authorwosidTuğ, Niyazi/HLH-6301-2023
dc.contributor.authorTug, Niyazi
dc.contributor.authorSandal, Suleyman
dc.contributor.authorOzelgun, Berna
dc.contributor.authorYilmaz, Bayram
dc.date.accessioned2024-08-04T20:32:40Z
dc.date.available2024-08-04T20:32:40Z
dc.date.issued2011
dc.departmentİnönü Üniversitesien_US
dc.description.abstractWe have investigated a relationship between DNA damage in sperm and spermiogram profiles in the infertile men. Twenty-one non-smoking infertile men <40 years of age with no systemic or genetic disease were randomly selected from the pool of infertile couples applied to our clinic. Sperm samples were collected and evaluated according to WHO guidelines. DNA damage of sperm cells was assessed using neutral comet assay. Fifty cells per slide and two slides per sample were scored to evaluate DNA damage. The cells were visually classified into four categories based on DNA migration such as undamaged (UD), little damage (LD), moderate damage (MD) and significant damage (SD). Total comet scores (TCS) were calculated as: 1 x UD + 2 x LD + 3 x MD + 4 x SD. There was a negative correlation between the percentage of slow-and in situ-motion sperm cells in spermiograms and TCS (p < 0.001 and p < 0.05, respectively). The relationship between the percentage of non-motile sperm cells and TCS was negative (p < 0.001). This study provides new evidence that DNA damage in spermatozoa and sperm motility parameters are negatively correlated. We suggest that evaluation of sperm DNA by the neutral comet assay may be valuable to use in fertility research.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [104-T-240]en_US
dc.description.sponsorshipThis study is supported by The Scientific and Technological Research Council of Turkey (TUBITAK project No. 104-T-240). We thank Dr. Gulhan Aktas of the Zeynep Kamil Maternity and Children's Hospital in Istanbul for her technical assistance in the Andrology Laboratory.en_US
dc.identifier.doi10.3109/09513590.2010.487598
dc.identifier.endpage54en_US
dc.identifier.issn0951-3590
dc.identifier.issn1473-0766
dc.identifier.issue1en_US
dc.identifier.pmid20504093en_US
dc.identifier.scopus2-s2.0-78650126883en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage49en_US
dc.identifier.urihttps://doi.org/10.3109/09513590.2010.487598
dc.identifier.urihttps://hdl.handle.net/11616/95213
dc.identifier.volume27en_US
dc.identifier.wosWOS:000285143600008en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofGynecological Endocrinologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSpermen_US
dc.subjectDNA damageen_US
dc.subjectinfertile menen_US
dc.subjectcomet assayen_US
dc.subjectspermiogramen_US
dc.titleCorrelation of spermiogram profiles with DNA damage in sperm cells of infertile men: a comet assay studyen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu