Pinealectomy alters ıfn-gamma and ıl-10 levels in primary thymocyte culture of rats

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dc.contributor.authorSahin, Zafer
dc.contributor.authorSandal, Suleyman
dc.contributor.authorYilmaz, Bayram
dc.contributor.authorBulmus, Ozgur
dc.contributor.authorOzdemir, Gokcen
dc.contributor.authorKutlu, Selim
dc.contributor.authorGodekmerdan, Ahmet
dc.contributor.authorKelestimur, Haluk
dc.date.accessioned2019-07-19T07:41:55Z
dc.date.available2019-07-19T07:41:55Z
dc.date.issued2018
dc.departmentİnönü Üniversitesien_US
dc.description.abstractMelatonin, produced mainly by the pineal gland, has an immunomodulatory role. However, the effects of the pineal gland and/or melatonin on thymus cytokine levels such as interferon-gamma (IFN-gamma), interleukin (IL)-4, and IL-10 are not well known. Twenty-one male Wistar rats (220-250 gr) were randomly divided into three groups (n=7): intact control, sham, and pinealectomy. Primary thymocyte cultures were prepared from each group and dispensed into well plates as Control, DMSO (or vehicle), Sham-pinealectomy, Pinealectomy, Pinealectomy+10 mu M melatonin, and Pinealectomy+100 mu M melatonin. IFN-gamma, IL-4, and IL-10 concentrations were measured in the thymocytes (as nonstimulated and Concanavalin A-stimulated) after 24 h. IFN-gamma levels significantly increased and IL-10 levels significantly decreased in both media prepared from pinealectomized rats. There was no significant difference between the groups in terms of IL-4. In the pinealectomy+100 mu M melatonin group, IFN-gamma and IL-10 levels did not differ from the pinealectomy group. However, the dose of 100 mu M melatonin caused a decrease in levels of IFN-gamma in both thymocyte media and an increase in the concentration of IL-10 in Concanavalin A-stimulated thymocytes. In conclusion, pineal gland and or melatonin affect IFN-gamma and IL-10 levels in the thymus gland.en_US
dc.identifier.citationSahin, Z. Sandal, S. Yilmaz, B. Bulmus, O. Ozdemir, G. Kutlu, S . Godekmerdan, A. Kelestimur, H. (2018). Cilt:64. Sayı:14. 25-30 ss.en_US
dc.identifier.doi10.14715/cmb/2018.64.14.5en_US
dc.identifier.endpage30en_US
dc.identifier.issue14en_US
dc.identifier.startpage25en_US
dc.identifier.urihttps://hdl.handle.net/11616/12779
dc.identifier.volume64en_US
dc.language.isoenen_US
dc.publisherC m b assoc, 34 boulevard solferıno, 86000 poıtıers, franceen_US
dc.relation.ispartofCellular and molecular bıologyen_US
dc.rightsinfo:eu-repo/semantics/restrictedAccessen_US
dc.subjectReceptor messenger-rnaen_US
dc.subjectt-cellsen_US
dc.subjectmelatonın synthesısen_US
dc.subjectın-vıtroen_US
dc.subjectthymusen_US
dc.subjectmouseen_US
dc.subjectexpressıonen_US
dc.subjectcytokınesen_US
dc.subjectlymphocytesen_US
dc.subjectmechanısmsen_US
dc.titlePinealectomy alters ıfn-gamma and ıl-10 levels in primary thymocyte culture of ratsen_US
dc.typeArticleen_US

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