Deneysel periodontitis oluşuturulan ratlarda rutin'in (vitamin P) alveoler kemik yıkımına ve çeşitli biyomarker seviyelerine etkisi
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2021
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İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Deneysel Periodontitis Oluşturulan Ratlarda Rutin'in (Vitamin P) Alveoler Kemik Yıkımına ve Çeşitli Biyomarker Seviyelerine Etkisi Amaç: Antioksidan ve antiinflamatuar özellikler gösteren Rutinin deneysel periodontitise etkisinin değerlendirilmesidir. Materyal ve Metot: 32 adet Wistar Albino erkek rat 4 eşit gruba ayrıldı; 1) SK, 2) DP, 3) R-50), 4) R-100. Ratların üst 2. molar dişlerine 4-0 ipek sütur bağlanarak deneysel periodontitis oluşturulduktan sonra R-50 ve R-100 gruplarına 14 gün boyunca oral gavaj yoluyla rutin verildi. 15. günde hayvanlar sakrifiye edildikten sonra dişeti ve maksilla örnekleri alındı. Dişeti dokusunda ELISA yöntemi ile IL-1β, IL-10, MDA, SOD, GPX, spektrofotometrik analiz ile TAS, TOS ve OSİ değerleri incelendi. Maksilla örneklerinde AKK, mikro-BT ile incelendi. Bulgular: Gruplar arasında IL-1β ve MDA değerlerinde anlamlı farklılık gözlenmedi (p>0.05). IL-1β/IL-10 oranı DP grubunda R-100 grubuna kıyasla anlamlı derecede yüksek bulundu (p<0.05). SOD seviyesi SK ve R-100 gruplarında DP grubuna göre anlamlı derecede yüksek bulundu (p<0.001). IL-10 ve GPX seviyesi R-100 grubunda DP grubuna kıyasla anlamlı derecede yüksek bulundu (p<0.05). TOS değeri DP grubunda SK grubuna kıyasla anlamlı derecede yüksek bulundu (p<0.05). TAS değeri SK grubunda R-50 grubuna göre anlamlı derecede yüksek bulundu (p<0.05). OSİ değeri DP grubunda diğer gruplara kıyasla anlamlı derecede yüksek bulundu (p<0.001). AKK DP grubunda SK grubuna göre, R-100 grubunda SK grubuna göre anlamlı derecede yüksek bulundu (p<0.001). R-50 grubu ile SK arasında istatistiksel anlamlı fark bulunmadı (p>0.05). Sonuç: Deneysel periodontitis modelinde Rutin kullanımının doza bağımlı olarak alveoler kemik yıkımını önleyebileceği ve inflamatuar/oksidatif parametreleri iyileştirebileceği sonucuna ulaşıldı. Rutinin doza bağlı etkilerini görebilmek için daha çok araştırmaya ihtiyaç olduğu sonucuna varıldı. Anahtar kelimeler: Alveoler kemik kaybı, glutatyon peroksidaz, interlökinler, malondialdehit, rutin, süperoksit dismutaz
The Effect of Rutin (Vitamin P) on Alveolar Bone Resorption and Various Biomarker Levels in Rats with Experimental Periodontitis Aim: The evaluation of the effect of rutin which shows antioxidant and antiinflammatory characteristics on experimental periodontitis. Material and Method: 32 Wistar Albino male rats were divided into 4 equal groups; 1) HC, 2) EP), 3) R-50, 4) R-100. After experimental periodontitis was created by ligating 4-0 silk sutures to the maxillary second molar teeth, rutin was given by oral gavage for 14 days. By ELISA method in gingival tissue; IL-1β, IL-10, MDA, SOD, GPX, Spectrophotometrically; TOS, TAS, OSI values were analyzed. ABL in maxillary specimens was examined by Micro-CT. Results: No significant difference was observed in IL-1β and MDA values (p>0.05). IL-1β/IL-10 ratio was found to be significantly higher in the EP group compared to the R-100 group (p<0.05). SOD level was higher in HC and R-100 groups than in EP group (p<0.001). IL-10 and GPX levels were found significantly higher in the R-100 group compared to the EP group (p<0.05). TOS values were found to be significantly higher in the EP group compared to the HC group (p<0.05). TAS values were found to be significantly higher in the HC group than in the R-50 group (p<0.05). OSI value was found to be significantly higher in the EP group compared to the other group (p<0.001). ABL values were found significantly higher in the EP group than in the HC group and in the R-100 group compared to the HC group (p<0.001). There was no statistically significant difference between the R-50 group and HC (p>0.05). Conclusions: It was concluded that rutin use in experimental periodontitis model can prevent alveolar bone destruction and improve inflammatory/oxidative parameters in a dose-dependent manner. It was concluded that more research is needed to see the doserelated effects of rutin. Keywords: Alveolar bone loss, glutathione peroxidase, interleukins, malondialdehyde, rutin, superoxide dismutase
The Effect of Rutin (Vitamin P) on Alveolar Bone Resorption and Various Biomarker Levels in Rats with Experimental Periodontitis Aim: The evaluation of the effect of rutin which shows antioxidant and antiinflammatory characteristics on experimental periodontitis. Material and Method: 32 Wistar Albino male rats were divided into 4 equal groups; 1) HC, 2) EP), 3) R-50, 4) R-100. After experimental periodontitis was created by ligating 4-0 silk sutures to the maxillary second molar teeth, rutin was given by oral gavage for 14 days. By ELISA method in gingival tissue; IL-1β, IL-10, MDA, SOD, GPX, Spectrophotometrically; TOS, TAS, OSI values were analyzed. ABL in maxillary specimens was examined by Micro-CT. Results: No significant difference was observed in IL-1β and MDA values (p>0.05). IL-1β/IL-10 ratio was found to be significantly higher in the EP group compared to the R-100 group (p<0.05). SOD level was higher in HC and R-100 groups than in EP group (p<0.001). IL-10 and GPX levels were found significantly higher in the R-100 group compared to the EP group (p<0.05). TOS values were found to be significantly higher in the EP group compared to the HC group (p<0.05). TAS values were found to be significantly higher in the HC group than in the R-50 group (p<0.05). OSI value was found to be significantly higher in the EP group compared to the other group (p<0.001). ABL values were found significantly higher in the EP group than in the HC group and in the R-100 group compared to the HC group (p<0.001). There was no statistically significant difference between the R-50 group and HC (p>0.05). Conclusions: It was concluded that rutin use in experimental periodontitis model can prevent alveolar bone destruction and improve inflammatory/oxidative parameters in a dose-dependent manner. It was concluded that more research is needed to see the doserelated effects of rutin. Keywords: Alveolar bone loss, glutathione peroxidase, interleukins, malondialdehyde, rutin, superoxide dismutase
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KESKİN, SAMİ B.(2021). Deneysel periodontitis oluşuturulan ratlarda rutin'in (vitamin P) alveoler kemik yıkımına ve çeşitli biyomarker seviyelerine etkisi,İnönü Üniversitesi / Diş Hekimliği Fakültesi / Periodontoloji Ana Bilim Dalı.
