The effects of methylene blue on lung injury in septic rats

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dc.authoridErsoy, Mehmet/0000-0002-0724-2825;
dc.authorwosidErsoy, Mehmet/ACN-2779-2022
dc.authorwosidKARAMAN, Abdurrahman/G-7825-2016
dc.authorwosidtürkmen, emine/GXH-9319-2022
dc.contributor.authorDemirbilek, S
dc.contributor.authorSizanli, E
dc.contributor.authorKaradag, N
dc.contributor.authorKaraman, A
dc.contributor.authorBayraktar, N
dc.contributor.authorTurkmen, E
dc.contributor.authorErsoy, MO
dc.date.accessioned2024-08-04T20:15:21Z
dc.date.available2024-08-04T20:15:21Z
dc.date.issued2006
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPurpose: We aimed to investigate the effects of methylene blue (MB) on NO production, myeloperoxidase (MPO) activity, antioxidant status and lipid peroxidation in lung injury during different stages of sepsis in rats. Material and Methods: Rats were randomly divided into 4 groups (n = 20): group C, sham operated; group CMB, sham operated and receiving MB (25 mg/kg, i.p.); group S, sepsis; group SMB, sepsis and receiving MB (25 mg/ kg, i.p.). Sepsis was induced by cecal ligation and puncture (CLP). The MB dose was administered after CLP. Each group was subdivided into two subgroups (n = 10) which were sacrificed at 9 or 18 h after the surgical procedure. The levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX) and MPO activity, total nitrite/nitrate and malondialdehyde (MDA) in the lung tissue were measured. Lung injury was graded from 1 (injury to 25% of the field) to 4 (diffuse injury) by the pathologist. Results: In group SMB, while SOD and CAT increased in both early and late sepsis periods, GSH-PX increased significantly only in the early sepsis period when compared with group S. Increase in lung MPO activity after CLP-induced sepsis was prevented by MB administration. MB significantly decreased to nitrite/nitrate and MDA levels both in early and late sepsis periods when compared with group S (p < 0.05). Group S showed a marked increase in neutrophil infiltration into the interstitial space and thickening of the alveolar septa, whereas the alveolar damage score was lower in the SMB group (p < 0.05). Conclusion: MB reduced the MPO activity and lipid peroxidation by both decreasing oxidative stress and NO overproduction in the lungs, which resulted in the attenuation of lung injury after CLP-induced sepsis in rats. Copyright (c) 2006 S. Karger AG, Basel.en_US
dc.identifier.doi10.1159/000091525
dc.identifier.endpage41en_US
dc.identifier.issn0014-312X
dc.identifier.issn1421-9921
dc.identifier.issue1en_US
dc.identifier.pmid16490992en_US
dc.identifier.scopus2-s2.0-33644912050en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage35en_US
dc.identifier.urihttps://doi.org/10.1159/000091525
dc.identifier.urihttps://hdl.handle.net/11616/94311
dc.identifier.volume38en_US
dc.identifier.wosWOS:000236127500007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherKargeren_US
dc.relation.ispartofEuropean Surgical Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectacute respiratory distress syndromeen_US
dc.subjectacute lung injuryen_US
dc.subjectneutrophil accumulationen_US
dc.subjectmethylene blueen_US
dc.subjectoxidative stressen_US
dc.titleThe effects of methylene blue on lung injury in septic ratsen_US
dc.typeArticleen_US

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