5-hidroksi indol asetik asitin poli (p-aminobenzen sülfonik asit) elektrotta miktarının belirlenmesi
Küçük Resim Yok
Tarih
2025
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, 5-hidroksiindolasetik asidin (5-HIAA) elektrokimyasal yükseltgenme özelliğinden yararlanılarak poli (p-aminobenzen sülfonik asit) (p-ABSA) modifiye camsı karbon elektrotta kantitatif miktarı saptandı. Analitik parametreleri optimize etmek için fosfat tamponunda Dönüşümlü voltametri (CV) ve diferansiyel puls voltametrisi (DPV) ile redoks özelliği incelendi. 5-HIAA için modifiye camsı karbon elektrotta keskin pikin ve maksimum akımın gözlendiği pH=7.00 fosfat tamponu destek elektrolit olarak seçildi. Pik akımına ve pik potansiyeline pH' ın etkisi diferansiyel puls voltametri tekniği ile tarama hızının pik akımına etkisi ise Dönüşümlü voltametri tekniği ile belirlendi. Alınan Dönüşümlü voltamogramlarda 5-HIAA'in redoks reaksiyonunun tersinmez olduğu görüldü. Dönüşümlü voltametri ile yapılan hız taraması çalışması, 5-HIAA'in redoks reaksiyonunun p-ABSA) modifiye camsı karbon elektrotta hem diffüzyon hem de adsorpsiyon kontrollü olduğunu göstermiştir. Bu nedenle, 5-HIAA kantitatif analizi için diferansiyel puls voltametrisi tekniği kullanıldı. Elde edilen kalibrasyon grafiklerinin doğrusal çalışma aralığı 1×10-5 – 9×10-5 M (korelasyon katsayısı 0.9912) ve tayin sınırı 5.3x10-7 M olarak belirlendi. İdrar örneğindeki analizlerinde geri kazanım değerleri % 99.4-% 103.0 arasında idi. Elde edilen sonuçlar, modifiye elektrodun askorbik asit varlığında 5-HIAA'in tayinine uygulanabileceğini göstermektedir. Önerilen sensör, yüksek seçiciliği, tekrarlanabilirliği, uzun vadeli kararlılık özellikleri ve biyolojik numunelerde elde edilen yüksek geri kazanım değerleri nedeniyle rutin analiz için umut vericidir.
In this study, the quantitative amount of 5-hydroxy indole acetic acid (5-HIAA) was determined by using the electrochemical oxidation property at poly (p-aminobenzene sulfonic acid) (p-ABSA) modified glassy carbon electrode. The redox property of 5-HIAA was investigated by cyclic voltammetry and differential pulse voltammetry to optimize the analytical parameters. Phosphate buffer solution at pH 7.00, in which a sharp peak and maximum current were observed at the modified glassy carbon electrode, was chosen as the supporting electrolyte for 5-HIAA. The effect of pH on the peak current and peak potential was determined by the differential pulse voltammetry technique, and the effect of scan rate on the peak current was determined by the cyclic voltammetry technique. The cyclic voltammograms showed that the redox reaction of 5-HIAA was irreversible. Scan rate study with cyclic voltammetry showed that the redox reaction of 5-HIAA was controlled by both diffusion and adsorption on the poly (p-ABSA) modified glassy carbon electrode. Differential pulse voltammetry technique was used for the quantitative analysis of 5-HIAA. The linear working range of the obtained calibration graphs was determined as 1×10-5 – 9×10-5 M (correlation coefficient 0.9912), and the detection limit was determined as 5.3x10-7 M. Recovery values in the analysis of urine samples were between 99.4% and 103.0%. The results showed that the modified electrode can be applied to the determination of 5-HIAA in the presence of ascorbic acid. The proposed sensor is promising for routine analysis due to its high selectivity, reproducibility, long-term stability, and high recovery values obtained in biological samples.
In this study, the quantitative amount of 5-hydroxy indole acetic acid (5-HIAA) was determined by using the electrochemical oxidation property at poly (p-aminobenzene sulfonic acid) (p-ABSA) modified glassy carbon electrode. The redox property of 5-HIAA was investigated by cyclic voltammetry and differential pulse voltammetry to optimize the analytical parameters. Phosphate buffer solution at pH 7.00, in which a sharp peak and maximum current were observed at the modified glassy carbon electrode, was chosen as the supporting electrolyte for 5-HIAA. The effect of pH on the peak current and peak potential was determined by the differential pulse voltammetry technique, and the effect of scan rate on the peak current was determined by the cyclic voltammetry technique. The cyclic voltammograms showed that the redox reaction of 5-HIAA was irreversible. Scan rate study with cyclic voltammetry showed that the redox reaction of 5-HIAA was controlled by both diffusion and adsorption on the poly (p-ABSA) modified glassy carbon electrode. Differential pulse voltammetry technique was used for the quantitative analysis of 5-HIAA. The linear working range of the obtained calibration graphs was determined as 1×10-5 – 9×10-5 M (correlation coefficient 0.9912), and the detection limit was determined as 5.3x10-7 M. Recovery values in the analysis of urine samples were between 99.4% and 103.0%. The results showed that the modified electrode can be applied to the determination of 5-HIAA in the presence of ascorbic acid. The proposed sensor is promising for routine analysis due to its high selectivity, reproducibility, long-term stability, and high recovery values obtained in biological samples.
Açıklama
Anahtar Kelimeler
Kimya, Chemistry
