Characterization of long living yeast deletion mutants that lack mitochondrial metabolism genes DSS1, PPA2 and AFG3

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dc.authoridKOÇ, AHMET/0000-0003-3484-2137
dc.authoridKimyon, Onder/0000-0001-9222-7672
dc.authorwosidKOÇ, AHMET/ABI-3322-2020
dc.authorwosidKimyon, Onder/J-8852-2014
dc.contributor.authorMuid, K. A.
dc.contributor.authorKimyon, Onder
dc.contributor.authorReza, Shahadat Hasan
dc.contributor.authorKarakaya, Huseyin Caglar
dc.contributor.authorKoc, Ahmet
dc.date.accessioned2024-08-04T20:45:56Z
dc.date.available2024-08-04T20:45:56Z
dc.date.issued2019
dc.departmentİnönü Üniversitesien_US
dc.description.abstractMolecular mechanisms of aging and longevity are still mostly unknown. Mitochondria play central roles in cellular metabolism and aging. In this study, we identified three deletion mutants of mitochondrial metabolism genes (ppa2 Delta, dss1 Delta, and afg3 Delta) that live longer than wild-type cells. These long-lived cells harbored significantly decreased amount of mitochondria] DNA (mtDNA) and reactive oxygen species (ROS). Compared to the serpentine nature of wild-type mitochondria, a different dynamics and distribution pattern of mitochondria were observed in the mutants. Both young and old long-lived cells produced relatively low but adequate levels of ATP for cellular activities. The status of the retrograde signaling was checked by expression of CIT2 gene and found activated in long-lived mutants. The mutant cells were also profiled for their gene expression patterns, and genes that were differentially regulated were determined. All long-lived cells comprised similar pleiotropic phenotype regarding mitochondrial dynamics and functions. Thus, this study suggests that DSS1, PPA2, and AFG3 genes modulate the lifespan by altering the mitochondrial morphology and functions.en_US
dc.description.sponsorshipTUBITAK [114Z983]; TUBITAK-BIDEPen_US
dc.description.sponsorshipThis work was supported by TUBITAK grant no 114Z983 to AK. MKA was supported by a predoctoral fellowship from the TUBITAK-BIDEP.en_US
dc.identifier.doi10.1016/j.gene.2019.05.001
dc.identifier.endpage180en_US
dc.identifier.issn0378-1119
dc.identifier.issn1879-0038
dc.identifier.pmid31082499en_US
dc.identifier.scopus2-s2.0-85065609536en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage172en_US
dc.identifier.urihttps://doi.org/10.1016/j.gene.2019.05.001
dc.identifier.urihttps://hdl.handle.net/11616/98793
dc.identifier.volume706en_US
dc.identifier.wosWOS:000472241500022en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofGeneen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLongevityen_US
dc.subjectAgingen_US
dc.subjectMitochondriaen_US
dc.subjectROSen_US
dc.subjectRespirationen_US
dc.subjectRetrograde signalingen_US
dc.subjectPPA2en_US
dc.subjectDSS1en_US
dc.subjectAFG3en_US
dc.titleCharacterization of long living yeast deletion mutants that lack mitochondrial metabolism genes DSS1, PPA2 and AFG3en_US
dc.typeArticleen_US

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