Moringa oleifera leaves: could solvent and extraction method affect phenolic composition and bioactivities?

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dc.authoridYesilada, Ozfer/0000-0003-0038-6575
dc.authoridKUCUKBAY, HASAN/0000-0002-7180-9486
dc.authoridBouhamda, Talel/0009-0004-6487-5583
dc.authorwosidYesilada, Ozfer/ABI-1335-2020
dc.authorwosidKUCUKBAY, HASAN/A-5050-2019
dc.authorwosidBouhamda, Talel/AAQ-6635-2021
dc.contributor.authorBennour, Naima
dc.contributor.authorMighri, Hedi
dc.contributor.authorBouhamda, Talel
dc.contributor.authorMabrouk, Mahmoud
dc.contributor.authorApohan, Elif
dc.contributor.authorYesilada, Ozfer
dc.contributor.authorKucukbay, Hasan
dc.date.accessioned2024-08-04T20:49:24Z
dc.date.available2024-08-04T20:49:24Z
dc.date.issued2021
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThis study was carried out to evaluate the effect of extraction methods (direct maceration (DM) and successive maceration (SM)) and solvents (dichloromethane (DCM), ethyl acetate (EAc), butanol (But), and aqueous extraction (Aqu)) on the phenolic composition and biological activities of Moringa oleifera leaves cultivated for the first time in Tunisia. Total polyphenol content (TPC), total flavonoid content (TFC) and LC-MS analysis were performed for all extracts. Antioxidant potential by DPPH, metal chelating, and FRAP assays, antibacterial activity against Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922) by the minimum inhibitory concentration method (MIC) and cytotoxic properties against lung cancer cells (A549), were determined. Phenolic compounds and biological activities of M. oleifera leaves depend on the method and the solvent used for the extraction of these bioactive substances. The But extract prepared by SM method exhibited the highest amounts of TPC (103.06 +/- 0.5 mg GAE/g DE) and showed the strongest potential antioxidant (CI50 = 0.26 mg/mL on DPPH test), antibacterial (MIC = 250 mu g/mL) and cytotoxic activities (GI(50) = 69.9 mu g/mL). LC-MS analysis showed that 28 phenolic compounds of 33 tested standards were found in M. oleifera leaves. The But extract obtained by SM method exhibited the highest amounts of rutin, quercetin and syringic acid.en_US
dc.identifier.doi10.1080/10826068.2021.1891550
dc.identifier.endpage1025en_US
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.issue10en_US
dc.identifier.pmid33729901en_US
dc.identifier.scopus2-s2.0-85102862418en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1018en_US
dc.identifier.urihttps://doi.org/10.1080/10826068.2021.1891550
dc.identifier.urihttps://hdl.handle.net/11616/99839
dc.identifier.volume51en_US
dc.identifier.wosWOS:000629917600001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPreparative Biochemistry & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiological activityen_US
dc.subjectextraction methoden_US
dc.subjectLC-MSen_US
dc.subjectMoringa oleiferaen_US
dc.subjectphenolic compoundsen_US
dc.titleMoringa oleifera leaves: could solvent and extraction method affect phenolic composition and bioactivities?en_US
dc.typeArticleen_US

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