In Vitro Dose Studies on Chitosan Nanoplexes for microRNA Delivery in Breast Cancer Cells

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dc.authoridŞALVA, EMINE/0000-0002-1159-5850
dc.authoridKaban, Kübra/0000-0001-7311-7187
dc.authoridSalva, Emine/0000-0002-1159-5850
dc.authorwosidŞALVA, EMINE/CAH-3062-2022
dc.authorwosidKaban, Kübra/JFB-1837-2023
dc.authorwosidSalva, Emine/ABI-2766-2020
dc.contributor.authorKaban, Kubra
dc.contributor.authorSalva, Emine
dc.contributor.authorAkbuga, Julide
dc.date.accessioned2024-08-04T20:42:56Z
dc.date.available2024-08-04T20:42:56Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractChanges in microRNA (miRNA) expression levels that play important roles in regulation lead to many pathological events such as cancer. The miR-200 family is an important target in cancer therapy. The aim of this study is to equilibrate endogenous levels between cancer and noncancerous cells to prevent serious side effects of miR-200c- and miR-141-like metastatic colonization. For the first time, the characterization of miR-200c and miR-141 cluster containing chitosan nanoplexes was shown, and the optimization of miRNA expression levels by conducting dose studies in breast cancer cell lines was made. The mean diameter of chitosan/miR-141 and chitosan/miR-200c nanoplexes ranged from 296 to 355 nm and from 294 to 380 nm depending on the N/P ratio, respectively. The surface charge of nanoplexes was positive with zeta potential of +12 to +26 mV. While naked miRNA was degraded after 0 min in a 10% serum-containing medium, chitosan/miRNA nanoplexes were protected for 72 h. During the in vitro cellular uptake study, nanoplexes were observed to be accumulating in the cytoplasm or nucleus. After using different doses for miR-200c, the determined doses are 750, 100, and 750 ng in the MCF-7, MDA-MB-231, and MDA-MB-435 cell lines, respectively. Doses were determined as 100 ng for MDA-MB-231 and 150 ng for MDA-MB-435 to reach endogenous miR-141 levels of MCF-10A. Our results suggest that chitosan nanoplexes for miR-200c and miR-141 are an efficient delivery system in terms of formulation and transfection. As a conclusion, dose studies are important to provide effective treatment with miRNAs.en_US
dc.description.sponsorshipMarmara University Scientific Research Projects Association [SAG-C-YLP-120514-0137]en_US
dc.description.sponsorshipThis study was supported by the Marmara University Scientific Research Projects Association (SAG-C-YLP-120514-0137). We would like to thank to Prof. Dr. Oguz Ozturk for kindly providing the MCF-10A cell line.en_US
dc.identifier.doi10.1089/nat.2016.0633
dc.identifier.endpage55en_US
dc.identifier.issn2159-3337
dc.identifier.issn2159-3345
dc.identifier.issue1en_US
dc.identifier.pmid27763825en_US
dc.identifier.scopus2-s2.0-85011347262en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage45en_US
dc.identifier.urihttps://doi.org/10.1089/nat.2016.0633
dc.identifier.urihttps://hdl.handle.net/11616/97682
dc.identifier.volume27en_US
dc.identifier.wosWOS:000393777800006en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofNucleic Acid Therapeuticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectdoseen_US
dc.subjectmiRNA therapeuticsen_US
dc.subjectchitosanen_US
dc.subjectbreasten_US
dc.subjectcanceren_US
dc.subjectmiR-200cen_US
dc.subjectmiR-141en_US
dc.titleIn Vitro Dose Studies on Chitosan Nanoplexes for microRNA Delivery in Breast Cancer Cellsen_US
dc.typeArticleen_US

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