Yüksek kolesterollü diyetle beslenen ratlardareyhan ekstraktı ve esansiyel yağının lipidmetabolizması üzerine etkileri
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2021
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info:eu-repo/semantics/openAccess
Özet
Hiperlipidemi, lipid metabolizması bozukluğuna bağlı gelişmekte olup, plazma lipoprotein ve trigliserid düzeyinin yükselmesi olarak ifade edilmektedir. Hiperlipidemi, damarların intima tabakası altında lipid birikimi sonucu, hücresel-humoral reaksiyonlara sebep olan ve ateroskleroz olarak adlandırılan vaskuler bozukluğa yol açmaktadır. Geleneksel bitkisel ilaçlar ve bunların preparatları, doğal kökenli olmaları ve daha az yan etkileri nedeniyle tedavi edici ve diğer farmakolojik etkilere sahip doğal ilaçlar olarak binlerce yıldır yaygın olarak kullanılmaktadır. Bu çalışmada, Malatya'nın Arapgir ilçesi'nde yetişen coğrafi işaret almış olan Arapgir mor reyhanının (Ocimum basilicum L.) hipolipidemik aktivitesinin araştırılması amaçlanmıştır. Çalışmada 70 adet Wistar Albino erkek rat kullanılmış ve 7 grup oluşturulmuştur. Kontrol, Kontrol + reyhan ekstraktı (200 mg/kg vücut ağırlığı), Kontrol + reyhan esansiyel yağı (70 mg/kg vücut ağırlığı), Kolesterol Kontrol, Kolesterol + reyhan ekstraktı (200 mg/kg vücut ağırlığı), Kolesterol + reyhan esansiyel yağı (70 mg/kg vücut ağırlığı) ve Kolesterol + fenofibrat (200 mg/kg vücut ağırlığı) şeklinde bir deneme dizaynı oluşturulmuştur. Çalışma sonunda analizler için tüm hayvanlardan kan ve karaciğer örnekleri alınmıştır. Ratların yüksek kolesterollü bir diyetle beslenmesi sonucunda, ratlarda serum lipid profillerini, leptini, hepatik trigliserit, kolesterol seviyelerini, malondialdehit (MDA) konsantrasyonlarını, FAS, LXR'ler, ACLY ve SREBP-1c karaciğerde ekspresyon seviyelerini artırmıştır. Ekstrakt ve uçucu yağ, serum trigliserit seviyelerini sırasıyla %28.2, %30.7 ve toplam kolesterolü %21.7 ve %14.5 oranında azaltmıştır. Uygulamalar, AST ve ALT aktivitelerini normal seviyelerine döndürememiştir. Ekstrakt ve fenofibrat uygulaması, serum leptin düzeylerini sırasıyla %17.5 ve %27.5 oranında azaltmıştır. Ekstrakt, hepatik trigliseriti %16.4 ve toplam kolesterolü %22.1 oranında azaltmıştır. Fenofibratın, trigliserit ve hepatik toplam kolesterol seviyelerini sırasıyla %25.5 ve %46.3 oranında azalttığı belirlenmiştir. MDA seviyelerindeki düşüş, ektrakt uygulananlarda %34.6, oranında gerçekleşmiştir. Karaciğerde FAS, LXR'ler, ACLY ve SREBP-1c ekspresyonu ekstrakt uygulandığında sırasıyla %20,9, %15,4, %20,7 ve %16.9 düzeyinde olmuştur. Uçucu yağ uygulananlar için düşüş %9.9, %13.6, %10.2 ve %12.5 olarak belirlenmiştir. Sonuç olarak, Arapgir mor reyhanının, yapısındaki fenolik bileşiklere bağlı olarak serum ve karaciğer lipid profilinde olumlu değişimlere yol açtığı ve kolesteol seviyesini düşürdüğü saptanmıştır. Anahtar kelimeler: Hiperlipidemi, Mor reyhan, Ekstrakt, Esansiyel yağ
Hyperlipidemia develops due to lipid metabolism disorder and is expressed as an increase in plasma lipoprotein and triglyceride levels. Hyperlipidemia causes a vascular disorder called atherosclerosis, which causes cellular-humoral reactions as a result of lipid accumulation under the intima layer of the vessels. Traditional herbal medicines and their preparations have been widely used for thousands of years as natural remedies with therapeutic and other pharmacological effects due to their natural origin and fewer side effects. In this study, it was aimed to investigate the hypolipidemic activity of purple basil (Ocimum basilicum L), grown in the Arapgir district of Malatya and received geographical indication. In the study, male 70 Wistar Albino rats were used and 7 groups were formed. Control, Control + basil extract (200 mg/kg body weight), Control + basil essential oil (70 mg/kg body weight), Cholesterol Control, Cholesterol + basil extract (200 mg/kg body weight), Cholesterol + basil essential oil (70 mg/kg body weight) and Cholesterol + fenofibrate (200mg/kg body weight) a trial design was created. At the end of the study, blood and liver samples were taken from all animals for analysis. Feeding a high- cholesterol diet increased serum lipid profiles, leptin, hepatic triglyceride, cholesterol levels, malondialdehyde (MDA) concentrations, FAS, LXRs, ACLY and SREBP-1c expression in the liver in rats. The extract and essential oil reduced serum triglycerides by 28.2%, 30.7%, and total cholesterol by 21.7% and 14.5%, respectively. The applications did not return the AST and ALT activities to their normal levels. Extract and fenofibrate administration decreased serum leptin levels by 17.5% and 27.5%, respectively. The extract reduced hepatic triglyceride by 16.4% and total cholesterol by 22.1%. Fenofibrate reduced the triglyceride and hepatic total cholesterol by 25.5% and 46.3%, respectively. While the decrease in MDA levels was 34.6% in the extract applied group, this rate was lower in the essential oil applied group. Expression of FAS, LXRs, ACLY and SREBP-1c in the liver decreased by 20.9%, 15.4%, 20.7% and 16.9%, respectively, when the extract was applied. For those treated with essential oil, the decline was 9.9%, 13.6%, 10.2%, and 12.5%. In conclusion, Arapgir purple basil causes positive changes in serum and liver lipid profile and lowering effect in cholesterol depending on the phenolic components in its structure. Keywords: Hyperlipidemia, Purple basil, Extract, Essential oil
Hyperlipidemia develops due to lipid metabolism disorder and is expressed as an increase in plasma lipoprotein and triglyceride levels. Hyperlipidemia causes a vascular disorder called atherosclerosis, which causes cellular-humoral reactions as a result of lipid accumulation under the intima layer of the vessels. Traditional herbal medicines and their preparations have been widely used for thousands of years as natural remedies with therapeutic and other pharmacological effects due to their natural origin and fewer side effects. In this study, it was aimed to investigate the hypolipidemic activity of purple basil (Ocimum basilicum L), grown in the Arapgir district of Malatya and received geographical indication. In the study, male 70 Wistar Albino rats were used and 7 groups were formed. Control, Control + basil extract (200 mg/kg body weight), Control + basil essential oil (70 mg/kg body weight), Cholesterol Control, Cholesterol + basil extract (200 mg/kg body weight), Cholesterol + basil essential oil (70 mg/kg body weight) and Cholesterol + fenofibrate (200mg/kg body weight) a trial design was created. At the end of the study, blood and liver samples were taken from all animals for analysis. Feeding a high- cholesterol diet increased serum lipid profiles, leptin, hepatic triglyceride, cholesterol levels, malondialdehyde (MDA) concentrations, FAS, LXRs, ACLY and SREBP-1c expression in the liver in rats. The extract and essential oil reduced serum triglycerides by 28.2%, 30.7%, and total cholesterol by 21.7% and 14.5%, respectively. The applications did not return the AST and ALT activities to their normal levels. Extract and fenofibrate administration decreased serum leptin levels by 17.5% and 27.5%, respectively. The extract reduced hepatic triglyceride by 16.4% and total cholesterol by 22.1%. Fenofibrate reduced the triglyceride and hepatic total cholesterol by 25.5% and 46.3%, respectively. While the decrease in MDA levels was 34.6% in the extract applied group, this rate was lower in the essential oil applied group. Expression of FAS, LXRs, ACLY and SREBP-1c in the liver decreased by 20.9%, 15.4%, 20.7% and 16.9%, respectively, when the extract was applied. For those treated with essential oil, the decline was 9.9%, 13.6%, 10.2%, and 12.5%. In conclusion, Arapgir purple basil causes positive changes in serum and liver lipid profile and lowering effect in cholesterol depending on the phenolic components in its structure. Keywords: Hyperlipidemia, Purple basil, Extract, Essential oil
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İnönü Üniversitesi Fen Bilimleri Enstitüsü
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GÖKÇE, Y. (2021). Yüksek kolesterollü diyetle beslenen ratlardareyhan ekstraktı ve esansiyel yağının lipidmetabolizması üzerine etkileri. Yayınlanmış Doktora Tezi, İnönü Üniversitesi.
