Tailor-made novel electrospun polycaprolactone/polyethyleneimine fiber membranes for laccase immobilization: An all-in-one material to biodegrade textile dyes and phenolic compounds

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dc.authoridUlu, Ahmet/0000-0002-4447-6233
dc.authoridBoran, Filiz/0000-0002-8801-7987
dc.authorwosidUlu, Ahmet/L-5180-2016
dc.contributor.authorKolak, Seda
dc.contributor.authorBirhanli, Emre
dc.contributor.authorBoran, Filiz
dc.contributor.authorBakar, Buesra
dc.contributor.authorUlu, Ahmet
dc.contributor.authorYesilada, Ozfer
dc.contributor.authorAtes, Burhan
dc.date.accessioned2024-08-04T20:53:15Z
dc.date.available2024-08-04T20:53:15Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIn spite of many works on the biodegradation of textile dyes and phenolic compounds, we propose a new, inexpensive, environmentally friendly, and sustainable material based on electrospun fiber and immobilized laccase. The polycaprolactone (PCL)/polyethyleneimine (PEI) electrospun fibers were optimized and prepared by electrospinning technique according to the operational parameters like PCL concentration (12 wt%), PEI con-centration (10 wt%), voltage (16 kV), needle tip-collector distance (20 cm), and injection speed (0.7 mL/h). Next, characterization studies were performed to investigate the morphology and structure of the electrospun fibers without and with laccase. The crude laccase was obtained by cultivating the white rot fungus T. trogii (TT), and T. versicolor (TV). The resulting electrospun fibers showed a smooth surface with a mean diameter of around 560 nm, and larger diameters were observed after laccase immobilization. According to the results, immobili-zation increased the stability properties of laccase such as storage, and operational. For instance, the residual activity of the PCL/PEI/TTL and PCL/PEI/TVL after 10 repeated cycles, was 33.2 & PLUSMN; 0.2% and 26.0 +/- 0.9%, respectively. After 3 weeks of storage, they retained around 30% of their original activity. Moreover, the PCL/ PEI/TTL and PCL/PEI/TVL were found to possess high decolorization yield to remove Orange II and Malachite Green textile dyes from solutions imitating polluted waters. Among them, the PCL/PEI/TTL exhibited the highest decolorization efficiencies of Orange II and Malachite Green after 8 continuous uses at pH 5 and a temperature of 50 C, reaching over 86%, and 46%, respectively. Moreover, PCL/PEI/TTL and PCL/PEI/TVL effectively degraded the 2,6-dichlorophenol phenolic compound at an optimal pH and temperature range and exhibited maximum removal efficiency of 52.6 +/- 0.1% and 64.5 +/- 7.6%, respectively. Our approach combines the ad-vantageous properties of electrospun fiber material and immobilization strategy for the efficient use of industrial scale important enzymes such as laccase in various enzymatic applications.en_US
dc.description.sponsorshipScientific Research Projects Unit of Inonu Universityen_US
dc.description.sponsorshipThis investigation was financially supported by the Scientific Research Projects Unit of Inonu University.en_US
dc.identifier.doi10.1016/j.chemosphere.2022.137478
dc.identifier.issn0045-6535
dc.identifier.issn1879-1298
dc.identifier.pmid36513203en_US
dc.identifier.scopus2-s2.0-85144019485en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.chemosphere.2022.137478
dc.identifier.urihttps://hdl.handle.net/11616/101060
dc.identifier.volume313en_US
dc.identifier.wosWOS:000904345100006en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofChemosphereen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectResponse-Surface Methodologyen_US
dc.subjectRepeated-Batch Cultureen_US
dc.subjectOrange Iien_US
dc.subjectDecolorizationen_US
dc.subjectRemovalen_US
dc.subjectSilicaen_US
dc.subjectEnzymeen_US
dc.subjectOptimizationen_US
dc.subjectNanofiberen_US
dc.subjectPelletsen_US
dc.titleTailor-made novel electrospun polycaprolactone/polyethyleneimine fiber membranes for laccase immobilization: An all-in-one material to biodegrade textile dyes and phenolic compoundsen_US
dc.typeArticleen_US

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