Population-based molecular epidemiological study of tuberculosis in Malatya, Turkey

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dc.authoridGünal, Selami/0000-0002-4752-5176
dc.authoridAllix-Béguec, Caroline/0000-0001-7642-4844
dc.authoridRASTOGI, NALIN/0000-0002-7199-7747
dc.authoridDURMAZ, RIZA/0000-0001-6561-778X
dc.authorwosidGünal, Selami/AAA-7246-2021
dc.authorwosidAllix-Béguec, Caroline/AAI-4645-2021
dc.authorwosidRASTOGI, NALIN/AAP-1958-2020
dc.authorwosidDURMAZ, Rıza/HJH-4918-2023
dc.contributor.authorDurmaz, Riza
dc.contributor.authorZozio, Thierry
dc.contributor.authorGunal, Selami
dc.contributor.authorAllix, Caroline
dc.contributor.authorFauville-Dufaux, Maryse
dc.contributor.authorRastogi, Nalin
dc.date.accessioned2024-08-04T20:30:38Z
dc.date.available2024-08-04T20:30:38Z
dc.date.issued2007
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThis investigation describes drug resistance patterns and genotyping data on a total of 145 Mycobacterium tuberculosis strains isolated between 2000 and 2004 in Malatya, Turkey. Drug susceptibility results indicated a total of 20% resistant and 4.8% of multidrug-resistant isolates. Spoligotyping resulted in 25 unique patterns and 120 strains in 19 clusters (2 to 33 strains per cluster). When the results were compared to an international spoligotyping database, 19 of 25 unique patterns matched existing shared spoligotype international types (SITs). This led to the description of 38 SITs with 139 strains and 6 orphan patterns (not previously reported). Five of the SITs (SIT759, SIT1936, SIT1937, SIT1938, and SIT2285) were newly created. The most prevalent spoligotype was SIT41 (LAM7-TUR) with 33 (23.9%) isolates. The repartition of strains according to major M. tuberculosis clades (in decreasing order) was as follows: ill-defined T clade (45.7%) > Latin American and Mediterranean (LAM; 29%) > Haarlem (15.9%). Strains belonging to Central Asian (CAS), East-African Indian (EAI), Beijing, and Africanum clades were absent in this setting. IS6110-restriction fragment length polymorphism (RFLP) resulted in 19 clusters (52 strains), with a final clustering rate of 35.9% and a recent transmission rate of 22.8%. Typing based on mycobacterial interspersed repetitive units (MIRUs) permitted us to identify 65 patterns (23 orphan patterns and 42 patterns that matched existing MIRU international types in an updated database). The combination of the three typing methods allowed us to calculate a final clustering rate of 22% and a significantly lower transmission rate of 13.1%. The discrimination achieved by IS6110-RFLP/MIRUs was not significantly improved by adding spolligotyping results (1.4%). We conclude that our patient population is infected by diverse M. tuberculosis populations; however, the majority of the ongoing transmission is due to evolutionary recent tuberculosis lineages belonging to principal genetic group 2 (PGG2; Haarlem and LAM) and PGG3 (ill-defined T clade), and most of it is attributable to the LAM7-TUR sublineage with an enhanced phylogeographical specificity for Turkey. An absence of lineages belonging to PGG1 clones (EAI, CAS, and Beijing, essentially found in Central, South, and Southeast Asia), is noteworthy.en_US
dc.identifier.doi10.1128/JCM.01308-07
dc.identifier.endpage4035en_US
dc.identifier.issn0095-1137
dc.identifier.issn1098-660X
dc.identifier.issue12en_US
dc.identifier.pmid17928426en_US
dc.identifier.scopus2-s2.0-37249002790en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage4027en_US
dc.identifier.urihttps://doi.org/10.1128/JCM.01308-07
dc.identifier.urihttps://hdl.handle.net/11616/94413
dc.identifier.volume45en_US
dc.identifier.wosWOS:000251630000026en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Soc Microbiologyen_US
dc.relation.ispartofJournal of Clinical Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMycobacterium-Tuberculosisen_US
dc.subjectDrug-Resistanceen_US
dc.subjectPulmonary Tuberculosisen_US
dc.subjectWestern Turkeyen_US
dc.subjectStrainsen_US
dc.subjectTransmissionen_US
dc.subjectPolymorphismen_US
dc.titlePopulation-based molecular epidemiological study of tuberculosis in Malatya, Turkeyen_US
dc.typeArticleen_US

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