Production of L-asparaginase in Enterobacter aerogenes expressing Vitreoscilla hemoglobin for efficient oxygen uptake

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dc.authoridGeckil, Hikmet/0000-0003-0070-0691
dc.authorwosidGeckil, Hikmet/F-7647-2012
dc.contributor.authorGeckil, H
dc.contributor.authorGencer, S
dc.date.accessioned2024-08-04T20:13:47Z
dc.date.available2024-08-04T20:13:47Z
dc.date.issued2004
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThis study is the first utilizing Vitreoscilla hemoglobin in a heterologous bacterium, Enterobacter aerogenes, to determine the effect of such a highly efficient oxygen-uptake system on the production of L-asparaginase, an enzyme that has attracted considerable attention due to its anti-tumor activity. Here, we show that the Vitreoscilla hemoglobin expressing strain has from 10-fold to more than two orders of magnitude lower L-asparaginase activity than the wild type or the control without the Vitreoscilla hemoglobin gene under different aeration conditions. Aeration and agitation were also determining factors for enzyme production. The enzyme activity was reduced considerably under both full aerobic and anaerobic conditions, while the highest enzyme activity was determined in cultures under low aeration and low agitation. Also, the effect of different concentrations of glucose on enzyme production showed catabolic repression. Glucose at 1% caused almost total inhibition of enzyme activity, while at 0.1% it showed a slightly stimulatory effect on enzyme production, compared with glucose-free medium.en_US
dc.identifier.doi10.1007/s00253-003-1482-5
dc.identifier.endpage697en_US
dc.identifier.issn0175-7598
dc.identifier.issue6en_US
dc.identifier.pmid14593509en_US
dc.identifier.scopus2-s2.0-1542269175en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage691en_US
dc.identifier.urihttps://doi.org/10.1007/s00253-003-1482-5
dc.identifier.urihttps://hdl.handle.net/11616/93844
dc.identifier.volume63en_US
dc.identifier.wosWOS:000189195300011en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer-Verlagen_US
dc.relation.ispartofApplied Microbiology and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEscherichia-Coli Asparaginaseen_US
dc.subjectPseudomonas-Aeruginosaen_US
dc.subjectSerratia-Marcescensen_US
dc.subjectPositive Regulationen_US
dc.subjectCulture Conditionsen_US
dc.subjectSequence-Analysisen_US
dc.subjectCell-Growthen_US
dc.subjectGlobin Geneen_US
dc.subjectCloningen_US
dc.subjectPromoteren_US
dc.titleProduction of L-asparaginase in Enterobacter aerogenes expressing Vitreoscilla hemoglobin for efficient oxygen uptakeen_US
dc.typeArticleen_US

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