Rapid Detection of Bloodstream Pathogens in Liver Transplantation Patients With Film Array Multiplex Polymerase Chain Reaction Assays: Comparison With Conventional Methods

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dc.authoridIsik, Burak/0000-0002-2395-3985
dc.authoridince, volkan/0000-0002-0714-490X
dc.authoridBayindir, Yasar/0000-0003-3930-774X
dc.authoridÖZDEMİR, FATİH/0000-0003-0292-3602
dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authoridOzdemir, Fatih/0000-0003-0292-3602
dc.authoridYilmaz, Sezai/0000-0002-8044-0297
dc.authorwosidIsik, Burak/A-6657-2018
dc.authorwosidÇuğlan, Serpil/JTU-9295-2023
dc.authorwosidince, volkan/M-7325-2017
dc.authorwosidBayindir, Yasar/T-1523-2017
dc.authorwosidYAKUPOGULLARI, YUSUF/F-3966-2011
dc.authorwosidÖZDEMİR, FATİH/HJI-7585-2023
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.contributor.authorOtlu, B.
dc.contributor.authorBayindir, Y.
dc.contributor.authorOzdemir, F.
dc.contributor.authorInce, V.
dc.contributor.authorCuglan, S.
dc.contributor.authorHopoglu, M.
dc.contributor.authorYakupogullari, Y.
dc.date.accessioned2024-08-04T20:41:07Z
dc.date.available2024-08-04T20:41:07Z
dc.date.issued2015
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground. Bloodstream infection (BSI) is an important concern in transplant patients. Early intervention with appropriate antimicrobial therapy is critical to better clinical outcome; however, there is significant delay when conventional identification methods are used. Methods. We aimed to determine the diagnostic performance of the FilmArray Blood Culture Identification Panel, a recently approved multiplex polymerase chain reaction assay detecting 24 BSI pathogens and 3 resistance genes, in comparison with the performances of conventional identification methods in liver transplant (LT) patients. A total of 52 defined sepsis episodes (signal-positive by blood culture systems) from 45 LT patients were prospectively studied. Results. The FilmArray successfully identified 37 of 39 (94.8%) bacterial and 3 of 3 (100%) yeast pathogens in a total of 42 samples with microbial growth, failing to detect only 2 of 39 (5.1%) bacterial pathogens that were not covered by the test panel. The FilmArray could also detect additional pathogens in 3 samples that had been reported as having monomicrobial growth, and it could detect Acinetobacter baumannii in 2 samples suspected of skin flora contamination. The remaining 8 blood cultures showing a positive signal but yielding no growth were also negative by this assay. Results of MecA, KPC, and VanA/B gene detection were in high accordance. The FilmArray produced results with significantly shorter turnaround times (1.33 versus 36.2, 23.6, and 19.5 h; P < .05) than standard identification methods, Vitek II, and Vitek MS, respectively. Conclusions. This study showed that the FilmArray appeared as a reliable alternative diagnostic method with the potential to mitigate problems with protracted diagnosis of the BSI pathogens in LT patients.en_US
dc.identifier.doi10.1016/j.transproceed.2015.02.025
dc.identifier.endpage1932en_US
dc.identifier.issn0041-1345
dc.identifier.issn1873-2623
dc.identifier.issue6en_US
dc.identifier.pmid26293075en_US
dc.identifier.scopus2-s2.0-84939442490en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1926en_US
dc.identifier.urihttps://doi.org/10.1016/j.transproceed.2015.02.025
dc.identifier.urihttps://hdl.handle.net/11616/96929
dc.identifier.volume47en_US
dc.identifier.wosWOS:000360776700065en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTransplantation Proceedingsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRisk-Factorsen_US
dc.subjectEtiologic Agentsen_US
dc.subjectIdentificationen_US
dc.subjectInfectionen_US
dc.subjectFilmarrayen_US
dc.subjectBacteremiaen_US
dc.subjectCulturesen_US
dc.subjectPerioden_US
dc.subjectTimeen_US
dc.titleRapid Detection of Bloodstream Pathogens in Liver Transplantation Patients With Film Array Multiplex Polymerase Chain Reaction Assays: Comparison With Conventional Methodsen_US
dc.typeArticleen_US

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