Identification of novel arsenic resistance genes in yeast

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dc.authoridTamas, Markus/0000-0002-0762-7848
dc.authorwosidTamas, Markus/E-8183-2010
dc.contributor.authorIsik, Esin
dc.contributor.authorBalkan, Cigdem
dc.contributor.authorKarl, Vivien
dc.contributor.authorKarakaya, Huseyin Caglar
dc.contributor.authorHua, Sansan
dc.contributor.authorRauch, Sebastien
dc.contributor.authorTamas, Markus J.
dc.date.accessioned2024-08-04T20:52:05Z
dc.date.available2024-08-04T20:52:05Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractArsenic is a toxic metalloid that affects human health by causing numerous diseases and by being used in the treatment of acute promyelocytic leukemia. Saccharomyces cerevisiae (budding yeast) has been extensively utilized to elucidate the molecular mechanisms underlying arsenic toxicity and resistance in eukaryotes. In this study, we applied a genomic DNA overexpression strategy to identify yeast genes that provide arsenic resistance in wild-type and arsenic-sensitive S. cerevisiae cells. In addition to known arsenic-related genes, our genetic screen revealed novel genes, including PHO86, VBA3, UGP1, and TUL1, whose overexpression conferred resistance. To gain insights into possible resistance mechanisms, we addressed the contribution of these genes to cell growth, intracellular arsenic, and protein aggregation during arsenate exposure. Overexpression of PHO86 resulted in higher cellular arsenic levels but no additional effect on protein aggregation, indicating that these cells efficiently protect their intracellular environment. VBA3 overexpression caused resistance despite higher intracellular arsenic and protein aggregation levels. Overexpression of UGP1 led to lower intracellular arsenic and protein aggregation levels while TUL1 overexpression had no impact on intracellular arsenic or protein aggregation levels. Thus, the identified genes appear to confer arsenic resistance through distinct mechanisms but the molecular details remain to be elucidated.en_US
dc.description.sponsorshipSvenska Forskningsradet Formas [942-2015-376]; Vetenskapsradet [348-2014-4296]en_US
dc.description.sponsorshipSvenska Forskningsradet Formas, Grant/Award Number: 942-2015-376; Vetenskapsradet, Grant/Award Number: 348-2014-4296en_US
dc.identifier.doi10.1002/mbo3.1284
dc.identifier.issn2045-8827
dc.identifier.issue3en_US
dc.identifier.pmid35765185en_US
dc.identifier.scopus2-s2.0-85132876893en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1002/mbo3.1284
dc.identifier.urihttps://hdl.handle.net/11616/100748
dc.identifier.volume11en_US
dc.identifier.wosWOS:000789144000001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofMicrobiologyopenen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectarsenateen_US
dc.subjectarseniteen_US
dc.subjectPho86en_US
dc.subjectTul1en_US
dc.subjectUgp1en_US
dc.subjectVba3en_US
dc.titleIdentification of novel arsenic resistance genes in yeasten_US
dc.typeArticleen_US

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