Calpain inhibitor AK 295 inhibits calpain-induced apoptosis and damage in rat kidney

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dc.authorscopusid57203284490
dc.authorscopusid57203279479
dc.authorscopusid6602664864
dc.authorscopusid6701329336
dc.contributor.authorAydemira S.
dc.contributor.authorBinena M.
dc.contributor.authorParlakpinar H.
dc.contributor.authorDilsiz N.
dc.date.accessioned2024-08-04T19:59:38Z
dc.date.available2024-08-04T19:59:38Z
dc.date.issued2018
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjectives: In the present investigation, the effect of a calpain inhibitor, AK 295, on ischemia/ reperfusion damage in rat kidney were investigated and evaluated. Materials and methods: 28 male Wistar Albino rats were separated into 4 main random groups (n=7), named group I (control), group II (ischemia-reperfusion), group III (AK295+ischemia-reperfusion) and group IV (DMSO+ischemia-reperfusion). Right kidney nephrectomy under anesthesia of xylazine (5 mg/kg) and ketamine (75 mg/kg) was performed to the rats except for group I. The rats under reversible general anesthesia underwent 30 min total ischemia and then, blood samples (5 cc) and kidney tissues were taken from all the groups rapidly. Caspase 3 enzyme activity was detected in the kidney tissues by Western-blot technique. Serum creatinine and urea values, amount of kidney malondialdehyde, and differences in the cellular antioxidant enzymes superoxide dismutase and catalase were also analyzed. Results: In AK295+IR group, caspase-3 enzyme band was not observed due to the fact that the calpain inhibitor AK295 prevented the ischemic damage. On the other hand, in DMSO+IR group, significant bands appeared due to apoptosis. It was also found that catalase levels in AK295+I/R and DMSO+I/R groups increased insignificantly compared to I/R group (p<0.05). Conclusions: As a result of the study, AK295 could provide further knowledge on ischemia-reperfusion damage that has no current treatment and it could have a place in ischemia-reperfusion damage treatment protocol. © 2018, Scientific Publishers of India. All rights reserved.en_US
dc.identifier.doi10.4066/biomedicalresearch.29-18-300
dc.identifier.endpage2521en_US
dc.identifier.issn0970-938X
dc.identifier.issue12en_US
dc.identifier.scopus2-s2.0-85051135605en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage2516en_US
dc.identifier.urihttps://doi.org/10.4066/biomedicalresearch.29-18-300
dc.identifier.urihttps://hdl.handle.net/11616/90765
dc.identifier.volume29en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherScientific Publishers of Indiaen_US
dc.relation.ispartofBiomedical Research (India)en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAK295en_US
dc.subjectApoptosisen_US
dc.subjectCalpainen_US
dc.subjectCaspase 3en_US
dc.subjectKidney ischemia-reperfusionen_US
dc.titleCalpain inhibitor AK 295 inhibits calpain-induced apoptosis and damage in rat kidneyen_US
dc.typeArticleen_US

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