Laccase production and dye decolorization by Trametes versicolor: application of Taguchi and Box-Behnken Methodologies

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dc.authoridYesilada, Ozfer/0000-0003-0038-6575
dc.authoridGedikli, Serap/0000-0001-5632-6585
dc.authorwosidApohan, Elif/AAA-5142-2021
dc.authorwosidÇelik, Pınar Aytar/AAB-4913-2020
dc.authorwosidBurnak, Nimetullah/KXQ-7910-2024
dc.authorwosidYesilada, Ozfer/ABI-1335-2020
dc.contributor.authorGedikli, Serap
dc.contributor.authorAytar, Pinar
dc.contributor.authorBuruk, Yeliz
dc.contributor.authorApohan, Elif
dc.contributor.authorCabuk, Ahmet
dc.contributor.authorYesilada, Ozfer
dc.contributor.authorBurnak, Nimetullah
dc.date.accessioned2024-08-04T20:39:58Z
dc.date.available2024-08-04T20:39:58Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjective: The aim of this study was to investigate the laccase production of Trametes versicolor under submerged fermentation condition. Then, dye decolorization by laccase was optimized using Box-Behnken methodology. Methods: The optimal culture conditions for producing high amount of laccase were determined using Taguchi methodology. The experiments were designed with five factors (glucose, yeast extract, CuSO4, inoculum size and pH) at three levels with orthogonal array layout of L27 (3(5)). Then, the optimum conditions for high decolorization activity of Reactive Blue 49 by obtained crude laccase were also investigated using Box-Behnken methodology. Results: The optimum culture conditions for production of high amounts of laccase were detected as 2 g L-1 of glucose, 5 g L-1 of yeast extract, 2mM of CuSO4, 4% of inoculum amount and pH 5.5. Yeast extract was the most effective factor, followed by CuSO4, inoculum, glucose and pH. Under these conditions, predicted values were in a good agreement with the actual experimental one. The predicted results showed that the maximum of Reactive Blue 49 decolorization as 98% could be obtained under the optimum conditions of pH 2.95, initial dye concentration 55.6 mg L-1, enzyme amount 0.76 mL and reaction time 46.91 min. The validity and practicability of this statistical optimization strategy was confirmed with the relation between predicted and experimental values. Conclusion: The results suggested that Taguchi method can be used in the optimization of laccase production process. Production of laccase by Trametes versicolor 2008001 can be effectively used for enzymatic decolorization according to the results of decolorization experiments in optimal levels.en_US
dc.identifier.doi10.5505/tjb.2014.62533
dc.identifier.endpage306en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-84912008715en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage298en_US
dc.identifier.urihttps://doi.org/10.5505/tjb.2014.62533
dc.identifier.urihttps://hdl.handle.net/11616/96604
dc.identifier.volume39en_US
dc.identifier.wosWOS:000346148200008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTurkish Biochem Socen_US
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLaccaseen_US
dc.subjectTaguchi Methoden_US
dc.subjectBox-Behnken Methodologyen_US
dc.subjectdye decolorizationen_US
dc.titleLaccase production and dye decolorization by Trametes versicolor: application of Taguchi and Box-Behnken Methodologiesen_US
dc.typeArticleen_US

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