Cape’nin Süperoksit Süpürücü Kapasitesinin In Vitro Incelenmesi
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Dosyalar
Tarih
2002
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi Tıp Fakültesi Dergisi
Erişim Hakkı
Attribution 3.0 United States
Özet
Son yillarda CAPE (Kafeik asit fenil esteri)’nin antioksidan kapasitesi arastirilmistir, ancak su ana
kadar yapilmis olan çalismalarin çogu in vivo çalismalardir. Biz bu çalismada CAPE’nin süperoksit
radikallerine karsi antioksidan kapasitesini in vitro arastirdik. CAPE bal arisi kovani propolisinin aktif
bir bileseni olup antimitojenik, antioksidan, antikarsinojen, antiinflamator ve immünomodülatör
özellikleri bilinmektedir.
Amaç: CAPE in vivo antioksidan bir moleküldür. Bu çalismada, CAPE’nin benzer kosullarda ancak in
vitro ortamda ayni etkiyi gösterip göstermediginin arastirilmasi amaçlandi.
Metod: McCord ve Fridovich’in SOD aktivitesi tayini için gelistirdikleri yöntem, SOD yerine CAPE
kullanilarak modifiye edildi.
Bulgular: CAPE’nin doza bagimli olarak artan bir süperoksit süpürücü kapasiteye sahip oldugunu
saptadik. 50 ?mol/L CAPE varliginda cyt c redüksiyonu %73,66 inhibe oldu.
The antioxidant capacity of CAPE (caffeic acid phenethyl ester) has been investigated in recent years but, so far most of the studies were in vivo studies. In this study we investigated, the in vitro antioxidant capacity of CAPE to scavenge superoxide radicals. CAPE, an active component of propolis from honeybee hives, is known to have anti mitogenic, antioxidant, anticarcinogenic, antiinflammatory and immunomodulatory properties. Aim of the study: CAPE is an antioxidant molecule in vivo. The aim of this study is to investigate if CAPE under in vitro conditions has the same effect similar to in vivo. Methods: The method developed by McCord and Fridovich for the determination of SOD activity is modified by replacing SOD with CAPE. Results: We find that, CAPE has superoxide scavenging capacity in a dose dependent manner. The presence of 50 μmol/L CAPE caused 73.66 % percentage of inhibition in cyt c reduction.
The antioxidant capacity of CAPE (caffeic acid phenethyl ester) has been investigated in recent years but, so far most of the studies were in vivo studies. In this study we investigated, the in vitro antioxidant capacity of CAPE to scavenge superoxide radicals. CAPE, an active component of propolis from honeybee hives, is known to have anti mitogenic, antioxidant, anticarcinogenic, antiinflammatory and immunomodulatory properties. Aim of the study: CAPE is an antioxidant molecule in vivo. The aim of this study is to investigate if CAPE under in vitro conditions has the same effect similar to in vivo. Methods: The method developed by McCord and Fridovich for the determination of SOD activity is modified by replacing SOD with CAPE. Results: We find that, CAPE has superoxide scavenging capacity in a dose dependent manner. The presence of 50 μmol/L CAPE caused 73.66 % percentage of inhibition in cyt c reduction.
Açıklama
Inönü Üniversitesi Tip Fakültesi Dergisi
9(1) 5-9 (2002)
Anahtar Kelimeler
CAPE, Süperoksit Süpürücü Kapasite, Oksidatif Stres
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Batçioglu, Kadir ;Öztürk, Çetin ;Inönü Üniversitesi Tip Fakültesi Dergisi 9(1) 5-9 (2002
