Development of besifloxacin HCl loaded nanofibrous ocular inserts for the treatment of bacterial keratitis: In vitro, ex vivo and in vivo evaluation

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dc.authoridCalamak, Semih/0000-0002-0989-6400
dc.authoridOzturk, Naile/0000-0002-7617-8433
dc.authoridOzkul, Ceren/0000-0002-0921-5863
dc.authoridOZKUL, CEREN/0000-0002-0921-5863
dc.authoridpolat, Heybet kerem/0000-0001-5006-3091
dc.authoridAYTEKIN, EREN/0000-0002-7146-326X
dc.authorwosidCALAMAK, SEMIH/I-8449-2013
dc.authorwosidCalamak, Semih/N-5698-2019
dc.authorwosidOzturk, Naile/F-3650-2017
dc.authorwosidOzkul, Ceren/AAA-9155-2020
dc.authorwosidOZKUL, CEREN/I-8445-2013
dc.authorwosidpolat, Heybet kerem/GYI-8812-2022
dc.contributor.authorPolat, H. Kerem
dc.contributor.authorPehlivan, Sibel Bozdag
dc.contributor.authorOzkul, Ceren
dc.contributor.authorCalamak, Semih
dc.contributor.authorOzturk, Naile
dc.contributor.authorAytekin, Eren
dc.contributor.authorFirat, Aysegul
dc.date.accessioned2024-08-04T20:47:25Z
dc.date.available2024-08-04T20:47:25Z
dc.date.issued2020
dc.departmentİnönü Üniversitesien_US
dc.description.abstractNovel drug delivery systems have emerged to treat bacterial keratitis, an acute infection of the cornea. In this study, besifloxacin HCl loaded insert formulations were designed and investigated in vitro, ex vivo and in vivo for the treatment of bacterial keratitis. Besifloxacin HCl (BH) or BH-hydroxypropyl-beta-cyclodextrin (HP-beta-CD) complex containing poly(caprolactone)/polyethylene glycol (PLC/PEG) fibrous inserts were prepared with an electrospinning method. These fibrous inserts were coated with mucoadhesive polymers such as sodium alginate (SA) or thiolated sodium alginate (TSA). Developed inserts compared to commercially available drug and it was found that coating of the insert surfaces with SA and TSA, increases bioadhesion of the formulations. Insert formulations showed a burst release in the first 2 days followed by a slow-release profile. Ex vivo transport studies showed that HP-beta-CD possessed a drug delivery level close to the commercial drug. Both TSA coated inserts as well as inserts containing HP-beta-CD-drug complex were effectively reducing bacterial keratitis in rabbit eyes upon single-dose application compared to multiple dosing with the commercial drug. Consequently, TSA coated inserts as well as the inserts containing HP-beta-CD-drug complex, may be potential alternatives to conventional market product by reducing the application frequency in the clinic leading to increased patient compliance.en_US
dc.description.sponsorshipThe Scientific and Technological Research Council of Turkey (TUBITAK) [SBAG217S126]en_US
dc.description.sponsorshipThis research was financially supported by The Scientific and Technological Research Council of Turkey (TUBITAK), (SBAG217S126).en_US
dc.identifier.doi10.1016/j.ijpharm.2020.119552
dc.identifier.issn0378-5173
dc.identifier.issn1873-3476
dc.identifier.pmid32569814en_US
dc.identifier.scopus2-s2.0-85086837656en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijpharm.2020.119552
dc.identifier.urihttps://hdl.handle.net/11616/99362
dc.identifier.volume585en_US
dc.identifier.wosWOS:000547385500012en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal of Pharmaceuticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectKeratitisen_US
dc.subjectOcular insertsen_US
dc.subjectBesifloxacinen_US
dc.subjectHydroxypropyl-beta-cyclodextrinen_US
dc.subjectThiolated sodium alginateen_US
dc.titleDevelopment of besifloxacin HCl loaded nanofibrous ocular inserts for the treatment of bacterial keratitis: In vitro, ex vivo and in vivo evaluationen_US
dc.typeArticleen_US

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