?-Amylase Immobilization on P(HEMA-co-PEGMA) Hydrogels: Preparation, Characterization, and Catalytic Investigation

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dc.authoridAteş, Burhan/0000-0001-6080-229X
dc.authoridUlu, Ahmet/0000-0002-4447-6233
dc.authorwosidAteş, Burhan/AAA-3730-2021
dc.authorwosidUlu, Ahmet/L-5180-2016
dc.contributor.authorDogan, Demet
dc.contributor.authorUlu, Ahmet
dc.contributor.authorSel, Evren
dc.contributor.authorKoytepe, Suleyman
dc.contributor.authorAtes, Burhan
dc.date.accessioned2024-08-04T20:50:14Z
dc.date.available2024-08-04T20:50:14Z
dc.date.issued2021
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe aims of this study are to synthesize and characterize poly (2-hydroxyethyl methacrylate-co-poly (ethylene glycol) methacrylate) (P(HEMA-co-PEG500MA)) structures containing polyethylene glycol (PEG) side groups and to investigate their possible use in alpha-amylase immobilization. For this purpose, P(HEMA-co-PEG500MA) copolymer structures are synthesized by using different monomer ratios. P(HEMA-co-PEG500MA) copolymer structures are confirmed by Fourier transform infrared spectroscopy (FTIR), and elemental analysis techniques. In addition, thermal, and morphological properties of the copolymers are investigated by thermal gravimetric analysis/differential scanning calorimetry, and scanning electron microscopy (SEM). Afterward, alpha-amylase from Aspergillus oryzae is immobilized on synthesized copolymer support by using physical interactions. The success of immobilization is elucidated via FTIR, SEM, and energy dispersive X-ray spectroscopy (EDX) methods. In addition, the influences of temperature, pH, storage time, and repeated uses on the activity of free and immobilized alpha-amylase are investigated. According to the outcomes, the immobilized alpha-amylase possesses a better pH and thermal resistance than the free one. Additionally, the immobilized alpha-amylase maintains about 53% of its original activity after eight reuses and it exhibits about 50% relative activity after 28 days of storage. In conclusion, the immobilized alpha-amylase can be utilized as a potential efficient catalyst to produce maltose from the hydrolysis of starch.en_US
dc.identifier.doi10.1002/star.202000217
dc.identifier.issn0038-9056
dc.identifier.issn1521-379X
dc.identifier.issue7-8en_US
dc.identifier.scopus2-s2.0-85105943076en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1002/star.202000217
dc.identifier.urihttps://hdl.handle.net/11616/99928
dc.identifier.volume73en_US
dc.identifier.wosWOS:000651011700001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofStarch-Starkeen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcopolymer sen_US
dc.subjectenzyme immobilizationen_US
dc.subjectimproved stabilityen_US
dc.subjectstarch hydrolysisen_US
dc.subject? ? amylaseen_US
dc.title?-Amylase Immobilization on P(HEMA-co-PEGMA) Hydrogels: Preparation, Characterization, and Catalytic Investigationen_US
dc.typeArticleen_US

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