Biophysical Model for Signal-Embedded Droplet Soaking into 2D Cell Culture
Küçük Resim Yok
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Association for Computing Machinery, Inc
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Using agar plates hosting a 2D cell population stimulated with signaling molecules is crucial for experiments such as gene regulation and drug discovery in a wide range of biological studies. In this paper, a biophysical model is proposed that incorporates droplet soaking, diffusion of molecules within agar, cell growth over an agar surface, and absorption of signaling molecules by cells. The proposed model describes the channel response and provides valuable insights for designing experiments more efficiently and accurately. The molecule release rate due to droplet soaking into agar, which is characterized and modeled as the source term for the diffusion model, is derived. Furthermore, cell growth is considered over the surface, which dictates the dynamics of signaling molecule reactions and leads to a variable boundary condition. As a case study, genetically-modified E. coli bacteria are spread over the surface of agar and Isopropyl-beta-D thiogalactopyranoside (IPTG) is considered as a signaling molecule. IPTG droplets are dropped onto the bacteria-covered agar surface. The parameters for the IPTG molecule release rate as a diffusion source into the agar are estimated from this experiment. Then, a particle-based simulator is used to obtain the spatio-temporal profile of the signaling molecules received by the surface bacteria. The results indicate that the number of molecules reacting with or absorbed by bacteria at different locations on the surface could be widely different, which highlights the importance of taking this variation into account for biological inferences. © 2023 Owner/Author.
Açıklama
NSF
10th ACM International Conference on Nanoscale Computing and Communication, NANOCOM 2023 -- 20 September 2023 through 22 September 2023 -- 192880
10th ACM International Conference on Nanoscale Computing and Communication, NANOCOM 2023 -- 20 September 2023 through 22 September 2023 -- 192880
Anahtar Kelimeler
agar plates, biophysical model, cell cultures, diffusion
Kaynak
NanoCom 2023 - Proceedings of the 10th ACM International Conference on Nanoscale Computing and Communication
WoS Q Değeri
Scopus Q Değeri
N/A
