Microarray analysis of cumulus cells in women with ovarian endometriosis undergoing intracytoplasmic sperm injection

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dc.authoridKaraer, Abdullah/0000-0002-2010-6211
dc.authoridDogan, Berat/0000-0003-4810-1970
dc.authoridCigremis, Yilmaz/0000-0002-8600-0946;
dc.authorwosidKaraer, Abdullah/ABI-4667-2020
dc.authorwosidDogan, Berat/AAJ-7288-2020
dc.authorwosidCigremis, Yilmaz/O-6019-2015
dc.authorwosidTuncay, Görkem/ABI-6418-2020
dc.authorwosidCigremis, Yilmaz/JAC-8451-2023
dc.contributor.authorKaraer, Abdullah
dc.contributor.authorTuncay, Gorkem
dc.contributor.authorDogan, Berat
dc.contributor.authorTecellioglu, Nihan
dc.contributor.authorCigremis, Yilmaz
dc.date.accessioned2024-08-04T20:47:12Z
dc.date.available2024-08-04T20:47:12Z
dc.date.issued2020
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjective: The aim of this study was to find the significantly altered genes in cumulus cells of women with ovarian endometriosis by using microarray and quantitative polymerase chain reaction analysis. Methods: Thirty women with ovarian endometriosis and 30 age-body mass index matched controls (women with infertility as a result of pure male factor) were enrolled in this study. Cumulus cells from study participants who underwent controlled ovarian hyperstimulation were isolated mechanically. Microarray comparative genomic hybridization was used to compare the transcriptome of cumulus cells from women with ovarian endometriosis and controls. According to the different expression levels in the microarrays and their putative functions, KRAS, ZNF322, and SDHA were selected and analyzed by real-time quantitative polymerase chain reaction. Results: There was no significant difference in the basal conditions between patients with endometriosis and controls, such as age, body mass index, basal follicle stimulating hormone and estradiol levels, and total gonadotrophin dosage. The gene expression profile of cumulus cells from patients with endometriosis was significantly different from that of controls. A total of 295 genes were significantly up- or down-regulated (p-value < 0.05 and absolute fold change > 1.5). For all of the genes adjusted p-value was found to be 0.999. Polymerase chain reaction analysis showed that KRAS and ZNF322 mRNA levels in the cumulus cells of patients with ovarian endometriosis were significantly up-regulated compared to controls (fold changes: 3.05 and 3.22, respectively). Conclusion: KRAS and ZNF322 mRNA levels in the cumulus cells of patients with ovarian endometriosis were significantly up-regulated.en_US
dc.description.sponsorshipTUBITAK (The Scientific and Technical Research Council of Turkey) [214S667]; Scientific Research Projects Unit of Inonu University [TSA-2018-1361]en_US
dc.description.sponsorshipThe author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by TUBITAK (The Scientific and Technical Research Council of Turkey) (Project number: 214S667) and Scientific Research Projects Unit of Inonu University (Project number: TSA-2018-1361).en_US
dc.identifier.doi10.1177/2284026520906070
dc.identifier.endpage93en_US
dc.identifier.issn2284-0265
dc.identifier.issn2284-0273
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85081616039en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage86en_US
dc.identifier.urihttps://doi.org/10.1177/2284026520906070
dc.identifier.urihttps://hdl.handle.net/11616/99223
dc.identifier.volume12en_US
dc.identifier.wosWOS:000523774200001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSage Publications Ltden_US
dc.relation.ispartofJournal of Endometriosis and Pelvic Pain Disordersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEndometriomaen_US
dc.subjectin vitro fertilizationen_US
dc.subjectKRASen_US
dc.subjectmicroarrayen_US
dc.subjectZNF322en_US
dc.titleMicroarray analysis of cumulus cells in women with ovarian endometriosis undergoing intracytoplasmic sperm injectionen_US
dc.typeArticleen_US

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