The effect of sustained and local administration of epidermal growth factor on improving bilateral testicular tissue after torsion

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dc.authoridHasirci, Nesrin/0000-0002-4497-0194
dc.authoridUĞURALP, SEMA/0000-0002-7628-0550
dc.authoridkaymaz, fevziye figen/0000-0001-8896-2471
dc.authoridbay karabulut, aysun/0000-0002-7873-2805
dc.authoridkiziltay, aysel/0000-0001-7515-2760
dc.authorwosidHasirci, Nesrin/I-4754-2018
dc.authorwosidUĞURALP, SEMA/ABH-6309-2020
dc.authorwosidkaymaz, fevziye figen/AGH-3508-2022
dc.authorwosidbay karabulut, aysun/HJP-0995-2023
dc.authorwosidkiziltay, aysel/ABI-2252-2020
dc.contributor.authorUguralp, S
dc.contributor.authorKarabulut, AB
dc.contributor.authorMizrak, B
dc.contributor.authorKaymaz, F
dc.contributor.authorKiziltay, A
dc.contributor.authorHasirci, N
dc.date.accessioned2024-08-04T20:56:13Z
dc.date.available2024-08-04T20:56:13Z
dc.date.issued2004
dc.departmentİnönü Üniversitesien_US
dc.description.abstractEpidermal growth factor (EGF) modulates Leydig cell proliferation, steroidogenesis, spermiogenesis, and Sertoli cell activity. It plays an important role in repairing ischemia-reperfusion injury in different tissues. The aim of this study was to evaluate the effects of sustained and local administration of EGF on improving bilateral testicular tissue after torsion. A total of 57 Wistar albino rats were used. For the EGF transport system, 1x2 cm gelatin films containing 2 mug EGF were used. Torsion was created by rotating the right testis 720degrees in a clockwise direction for 4 h in all groups except the control group. Then, in the torsion group, bilateral orchiectomy was performed. After returning the torsioned ipsilateral testes to their normal state, the bilateral testes were wrapped by 1x2 cm unloaded gelatin films in the gelatin (G7 and G21) groups and, by 2 mug EGF loaded gelatin films in the EGF 7 and EGF 21 groups. The testes were removed on the seventh and 21st days, respectively, for biochemical and histological examination. Histologically, Johnsen's spermatogenesis criteria and mean seminiferous tubule diameter (MSTD) measurements were used. The EGF7 group did not show significant loss of Sertoli cells, while in the G7 group the number of these cells decreased. The ipsilateral ischemic testis of the EGF21 group showed Leydig cell hyperplasia, and the contralateral non-ischemic testes in this group were similar to the control group. In the G21 group, the bilateral testes showed Sertoli cell only syndrome in some sections, and most of the cells were undergoing apoptosis. The mean spermatogenesis scores and MSTD in the EGF7 and EGF21 groups were higher than in the G7 and G21 groups (P<0.05). Malondialdehyde levels were significantly lower in the EGF groups than in the G groups (P<0.05). Glutathione peroxidase (GSH-Px) levels in the G21 group were significantly higher than in the EGF21 group. Our study shows that local and sustained EGF release after testicular torsion improves bilateral testicular injury. EGF administration may be a new treatment choice for bilaterally injured testis after detorsion without removing the twisted testis.en_US
dc.identifier.doi10.1007/s00240-004-0418-6
dc.identifier.endpage331en_US
dc.identifier.issn0300-5623
dc.identifier.issue5en_US
dc.identifier.pmid15338084en_US
dc.identifier.scopus2-s2.0-8744222324en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage323en_US
dc.identifier.urihttps://doi.org/10.1007/s00240-004-0418-6
dc.identifier.urihttps://hdl.handle.net/11616/102134
dc.identifier.volume32en_US
dc.identifier.wosWOS:000224754200003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofUrological Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectepidermal growth factoren_US
dc.subjecttestisen_US
dc.subjecttorsionen_US
dc.subjectischemiaen_US
dc.subjectreperfusionen_US
dc.subjecttreatmenten_US
dc.titleThe effect of sustained and local administration of epidermal growth factor on improving bilateral testicular tissue after torsionen_US
dc.typeArticleen_US

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