tRNA Wobble Base Modifications and Boric Acid Resistance in Yeast: Boron-Resistant Deletion Mutants Induce the General Amino Acid Control Mechanism and Activate Boron Efflux

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dc.authoridKOÇ, AHMET/0000-0003-3484-2137
dc.authorwosidKOÇ, AHMET/ABI-3322-2020
dc.contributor.authorUluisik, I.
dc.contributor.authorKarakaya, H. C.
dc.contributor.authorKoc, A.
dc.date.accessioned2024-08-04T20:47:24Z
dc.date.available2024-08-04T20:47:24Z
dc.date.issued2020
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBoric acid is essential for plants and has many vital roles in animals and microorganisms. However, its high doses are toxic to all organisms. We previously screened yeast deletion collections to identify boric acid-resistant and susceptible mutants to identify genes that play a role in boron tolerance. Here, we analyzed boron resistant mutants (elp1 increment ,elp3 increment ,elp6 increment ,ncs2 increment ,ncs6 increment andkti12 increment ) for their abilities to modulate the general amino acid control system (GAAC) and to induce boron efflux pumpATR1. The mutants analyzed in this study lack the genes that play roles in tRNA Wobble base modifications. We found that all of the boron resistant mutants activated Gcn4-dependent reporter gene activity and increased the transcript level of theATR1gene. Additionally, boron resistant cells accumulated less boric acid in their cytoplasm compared to the wild type cells upon boron exposure. Thus, our findings suggested that loss of wobble base modifications in tRNA leads to GAAC activation andATR1induction, which in turn reduced intracellular boron levels and caused boron resistance.en_US
dc.description.sponsorshipTurkish Scientific and Research Council (TuBTAK) [110T917]en_US
dc.description.sponsorshipThis work was supported by the Turkish Scientific and Research Council (TuBTAK) Grant number 110T917 to AK.en_US
dc.identifier.doi10.1134/S0026893320030188
dc.identifier.endpage401en_US
dc.identifier.issn0026-8933
dc.identifier.issn1608-3245
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85086576631en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage396en_US
dc.identifier.urihttps://doi.org/10.1134/S0026893320030188
dc.identifier.urihttps://hdl.handle.net/11616/99354
dc.identifier.volume54en_US
dc.identifier.wosWOS:000540780400010en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMaik Nauka/Interperiodica/Springeren_US
dc.relation.ispartofMolecular Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectboronen_US
dc.subjectboric aciden_US
dc.subjecttRNAen_US
dc.subjectWobble baseen_US
dc.subjectboron resistanceen_US
dc.subjectelongator complexen_US
dc.subjectyeasten_US
dc.subjectgeneral amino acid controlen_US
dc.titletRNA Wobble Base Modifications and Boric Acid Resistance in Yeast: Boron-Resistant Deletion Mutants Induce the General Amino Acid Control Mechanism and Activate Boron Effluxen_US
dc.typeArticleen_US

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