Comparison of VEGF gene silencing efficiencies of chitosan and protamine complexes containing shRNA

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dc.authoridSalva, Emine/0000-0002-1159-5850
dc.authoridŞALVA, EMINE/0000-0002-1159-5850
dc.authorwosidSalva, Emine/ABI-2766-2020
dc.authorwosidŞALVA, EMINE/CAH-3062-2022
dc.contributor.authorErdem-Cakmak, Fulden
dc.contributor.authorOzbas-Turan, Suna
dc.contributor.authorSalva, Emine
dc.contributor.authorAkbuga, Julide
dc.date.accessioned2024-08-04T20:39:53Z
dc.date.available2024-08-04T20:39:53Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractVEGF is an angiogenic factor promoting the proliferation and migration of endothelial cells. Inhibition of VEGF by RNAi mechanism is one of the novel and the most important strategies in antiangiogenesis therapy. In this study, the tumor silencing efficiency of ternary complexes after addition of protamine to chitosan complexes containing VEGF targeting shRNA was investigated. Besides chitosan, protamine is an effective gene delivery material. Binary and ternary complexes consisting of chitosan, protamine, and shRNA were prepared to target VEGF, their morphology, size, and zeta potential of the complexes being measured. The average size of the complexes was between 173 and 284nm and zeta potential was between +10 and 16mV. In the ternary complexes, size decreased as the chitosan ratio increased; however, its molecular weight had no effect on the size of complexes. HeLa, HEK293, and MCF-7 cell lines were used for in vitro transfection. VEGF was assayed by ELISA. A higher silencing effect was obtained using ternary complexes. Transgene expression was increased by adding protamine to chitosan complexes. Gene inhibition values in cell lines followed the rank HEK293>HeLa>MCF-7. The addition of protamine to the chitosan/shRNA (VEGF) complexes increased the knockdown of VEGF genes in the cell lines, and no cytotoxicity was found after the complexes had been incorporated into the cells.en_US
dc.description.sponsorshipMarmara University Scientific Research Projects Association [SAG-D-110412-0094]en_US
dc.description.sponsorshipThis study was supported by Marmara University Scientific Research Projects Association (SAG-D-110412-0094).en_US
dc.identifier.doi10.1002/cbin.10317
dc.identifier.endpage1270en_US
dc.identifier.issn1065-6995
dc.identifier.issn1095-8355
dc.identifier.issue11en_US
dc.identifier.pmid24890139en_US
dc.identifier.scopus2-s2.0-84908238474en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1260en_US
dc.identifier.urihttps://doi.org/10.1002/cbin.10317
dc.identifier.urihttps://hdl.handle.net/11616/96579
dc.identifier.volume38en_US
dc.identifier.wosWOS:000342798200005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofCell Biology Internationalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectshort hairpin RNAen_US
dc.subjectVEGFen_US
dc.subjectchitosanen_US
dc.subjectprotamineen_US
dc.subjectcomplexen_US
dc.titleComparison of VEGF gene silencing efficiencies of chitosan and protamine complexes containing shRNAen_US
dc.typeArticleen_US

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