Detection and Molecular Characterization of two 'Candidatus Phytoplasma Trifolii' Isolates Infecting Peppers at the Same Ecological Niche

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dc.authoridOKSAL, Erçin/0000-0002-7049-4419
dc.authoridOKSAL, Hatice Diğdem/0000-0003-1189-7864
dc.authoridKAYA APAK, Fulya/0000-0001-9226-6583;
dc.authorwosidOksal, Erçin/W-2507-2019
dc.authorwosidOKSAL, Erçin/AAH-5684-2019
dc.authorwosidOKSAL, Hatice Diğdem/AAW-1128-2020
dc.authorwosidKAYA APAK, Fulya/JCD-9921-2023
dc.authorwosidOksal, Erçin/ACH-2341-2022
dc.authorwosidTursun, Nihat/AID-5028-2022
dc.authorwosidTursun, Nihat/AAA-4957-2020
dc.contributor.authorOksal, Hatice Digdem
dc.contributor.authorApak, Fulya Kaya
dc.contributor.authorOksal, Ercin
dc.contributor.authorTursun, Nihat
dc.contributor.authorSipahioglu, Hikmet Murat
dc.date.accessioned2024-08-04T20:44:06Z
dc.date.available2024-08-04T20:44:06Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPepper (Capsicum annuum L.) cultivars exhibiting phytoplasma like symptoms including yellowing, flower sterility, necrosis, stunting and small leaves of lateral shoots were collected in Spring 2016 from Malatya province (Turkey). Leaf samples of the most common annual weeds and leafhoppers nearby symptomatic peppers were also sampled. Nested-PCR and virtual computer-simulated restriction fragment length polymorphism (virtual RFLP) methods have been implemented to ascertain and characterize the phytoplasma-associated disease. Using universal primer pairs in nested-PCR DNA fragments of approximately 1.2 kb were amplified from 3 pepper samples. None of the weed and leafhopper samples were reacted positive in PCR reactions. Next-generation sequencing (NGS) was used to sequence the amplified PCR fragments of two samples. The presence of 'Candidatus Phytoplasma trifolii' infections were confirmed by the analysis of 16S rDNA sequence and virtual-RFLP. Molecularly characterized isolates were designated as TR1 and TR2 (Acces. no: KY321932 and KY568694). Both isolates were identified as members of the clover proliferation phytoplasma group (subgroup 16SrVI-A) in pepper plants as strains of 'Ca. Phytoplasma trifolii'. Sequence alignment of the two 'Ca. Phytoplasma trifolii' isolates revealed a low level of genetic diversity. However, the restriction enzyme patterns of both isolates particularly in MseI profiles were differed from reference patterns of all previously established 'Ca. Phytoplasma trifolii' isolates in the world. Particularly, the TR2 isolate showed a point mutation comparing TR1 isolate and with reference strain (AY390261) of 'Ca. Phytoplasma trifolii'. This is the first report of 'Ca. Phytoplasma trifolii' isolates naturally infecting pepper plants from Turkey. (C) 2017 Friends Science Publishersen_US
dc.identifier.doi10.17957/IJAB/15.0420
dc.identifier.endpage1378en_US
dc.identifier.issn1560-8530
dc.identifier.issn1814-9596
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85034069791en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1372en_US
dc.identifier.urihttps://doi.org/10.17957/IJAB/15.0420
dc.identifier.urihttps://hdl.handle.net/11616/98029
dc.identifier.volume19en_US
dc.identifier.wosWOS:000416107100011en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherFriends Science Publen_US
dc.relation.ispartofInternational Journal of Agriculture and Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPepperen_US
dc.subject'Ca. phytoplasma trifolii'en_US
dc.subjectMolecular characterizationen_US
dc.subjectTurkeyen_US
dc.titleDetection and Molecular Characterization of two 'Candidatus Phytoplasma Trifolii' Isolates Infecting Peppers at the Same Ecological Nicheen_US
dc.typeArticleen_US

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