A novel murine model of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) induced by immunization with a spermine binding protein (p25) peptide

Basit Öğe Kaydı
dc.authoridBakhautdin, Bakytzhan/0000-0001-9486-6814
dc.authoridIZGI, Kenan/0009-0002-2402-5360
dc.contributor.authorAltuntas, Cengiz Z.
dc.contributor.authorDaneshgari, Firouz
dc.contributor.authorVeizi, Elias
dc.contributor.authorIzgi, Kenan
dc.contributor.authorBicer, Fuat
dc.contributor.authorOzer, Ahmet
dc.contributor.authorGrimberg, Kerry O.
dc.date.accessioned2024-08-04T20:37:34Z
dc.date.available2024-08-04T20:37:34Z
dc.date.issued2013
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAltuntas CZ, Daneshgari F, Veizi E, Izgi K, Bicer F, Ozer A, Grimberg KO, Bakhautdin B, Sakalar C, Tasdemir C, Tuohy VK. A novel murine model of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) induced by immunization with a spermine binding protein (p25) peptide. Am J Physiol Regul Integr Comp Physiol 304: R415-R422, 2013. First published January 23, 2013; doi: 10.1152/ajpregu.00147.2012.-The pathophysiology of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is poorly understood. Inflammatory and autoimmune mechanisms may play a role. We developed a murine model of experimental autoimmune prostatitis (EAP) that mimics the human phenotype of CP/CPPS. Eight-week- old mice were immunized subcutaneously with prostate-specific peptides in an emulsion of complete Freund's adjuvant. Mice were euthanized 10 days after immunization, and lymph node cells were isolated and assessed for recall proliferation to each peptide. P25 99-118 was the most immunogenic peptide. T-cell and B-cell immunity and serum levels of C-reactive protein and nitrate/nitrite levels were evaluated over a 9-wk period. Morphometric studies of prostate, 24-h micturition frequencies, and urine volume per void were evaluated. Tactile referred hyperalgesia was measured using von Frey filaments to the pelvic region. The unpaired Student's t-test was used to analyze differences between EAP and control groups. Prostates from p25 99-118-immunized mice demonstrated elevated gene expression levels of TNF-alpha, IL-17A, IFN-gamma, and IL-1 beta, not observed in control mice. Compared with controls, p25 99-118-immunized mice had significantly higher micturition frequency and decreased urine output per void, and they demonstrated elevated pelvic pain response. p25 99-118 immunization of male SWXJ mice induced prostate-specific autoimmunity characterized by prostate-confined inflammation, increased micturition frequency, and pelvic pain. This autoimmune prostatitis model provides a useful tool for exploring the pathophysiology and new treatments.en_US
dc.description.sponsorshipNational Institutes of Health [R-03-HD-061825, R-01-CA-14035]en_US
dc.description.sponsorshipThis work was supported by National Institutes of Health Grant R-03-HD-061825 (to F. Daneshgari) and R-01-CA-14035 (to V. K. Tuohy).en_US
dc.identifier.doi10.1152/ajpregu.00147.2012
dc.identifier.endpageR422en_US
dc.identifier.issn0363-6119
dc.identifier.issn1522-1490
dc.identifier.issue6en_US
dc.identifier.pmid23344231en_US
dc.identifier.scopus2-s2.0-84877150946en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpageR415en_US
dc.identifier.urihttps://doi.org/10.1152/ajpregu.00147.2012
dc.identifier.urihttps://hdl.handle.net/11616/96048
dc.identifier.volume304en_US
dc.identifier.wosWOS:000316195200003en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Physiological Socen_US
dc.relation.ispartofAmerican Journal of Physiology-Regulatory Integrative and Comparative Physiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectanimal modelen_US
dc.subjectchronic pelvic pain syndromeen_US
dc.subjectexperimental autoimmune prostatitisen_US
dc.subjectspermine binding protein (p25)en_US
dc.titleA novel murine model of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) induced by immunization with a spermine binding protein (p25) peptideen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu