Cytological and cytomorphometric characteristics of buccal mucosa cells from smokeless tobacco users

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dc.authoridAKATLI, AYSE NUR/0000-0002-9677-2456
dc.authoridBozdag, Zehra/0000-0002-0477-2513
dc.authoridDalkilic, Semih/0000-0002-6892-247X
dc.authoridDagli, Adile Ferda/0000-0003-4077-4134
dc.authoridAKKOC, Ramazan Fazil/0000-0002-0559-8932
dc.authorwosidAKATLI, AYSE NUR/ABH-4455-2020
dc.authorwosidBozdag, Zehra/AAG-5279-2020
dc.authorwosidŞahin, İbrahim/HHY-8303-2022
dc.authorwosidDalkilic, Semih/V-8138-2018
dc.authorwosidDagli, Adile Ferda/W-7878-2018
dc.authorwosidDalkılıç, Semih/GLV-3548-2022
dc.authorwosidartaş, gökhan/HTR-8629-2023
dc.contributor.authorDagli, Adile Ferda
dc.contributor.authorSahin, Nurhan
dc.contributor.authorBozdag, Zehra
dc.contributor.authorUcer, Ozlem
dc.contributor.authorAkatli, Ayse Nur
dc.contributor.authorArtas, Gokhan
dc.contributor.authorSahin, Ibrahim
dc.date.accessioned2024-08-04T20:43:58Z
dc.date.available2024-08-04T20:43:58Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackgroundUse of smokeless tobacco (ST) is increasing in many communities. We investigated whether ST alters the cytological and cytomorphometric features of buccal mucosa cells. MethodsTwenty male participants who had used Nicotiana rustica Linn.-containing ST (Maras powder) for at least 10 years, and 20 healthy male controls who did not use ST, were included in this study. After rinsing the mouth with water, samples were taken using a toothbrush from the buccal mucosa of subjects in both groups. Samples were gently spread over a glass slide. After applying a cytofixative spray, the Papanicolaou method was used to stain the slides. The presence of dysplasia, dyskeratosis, parakeratosis, hyperkeratosis, hypergranulosis, karyorrhexis, and pyknosis was evaluated by light microscopy, as were the increment amount of candida, cocco-bacillus, and Leptotrichia buccalis. Cytomorphometric analysis was performed and at least 20 cells with well-defined borders were evaluated from each slide, and the cellular diameter (CD), nuclear diameter (ND), and nucleus/cytoplasm (N/C) ratio of the cells were analyzed using a 60x objective. ResultsOther than the presence of dysplasia and candida, all measured cytological parameters were significantly higher in the ST users than in the non-ST users. Furthermore, CD was lower while nuclear/cytoplasmic ratio was higher in the ST users than in those non-ST users. ConclusionCytological changes associated with the use of ST, include dyskeratosis, parakeratosis, hyperkeratosis, hypergranulosis, karyorrhexis, pyknosis together with increase in the bacterial population of cocco-bacillus and L. buccalis. There were no significant differences in patients with dysplasia in spite of reduction of CD, increased nuclear size and N/C ratio.en_US
dc.identifier.doi10.1002/dc.23803
dc.identifier.endpage982en_US
dc.identifier.issn8755-1039
dc.identifier.issn1097-0339
dc.identifier.issue11en_US
dc.identifier.pmid28862802en_US
dc.identifier.scopus2-s2.0-85028816859en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage976en_US
dc.identifier.urihttps://doi.org/10.1002/dc.23803
dc.identifier.urihttps://hdl.handle.net/11616/97958
dc.identifier.volume45en_US
dc.identifier.wosWOS:000412681800003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofDiagnostic Cytopathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbuccal mucosaen_US
dc.subjectcellular diameteren_US
dc.subjectcytologyen_US
dc.subjectcytomorphometricsen_US
dc.subjectMaras powderen_US
dc.subjectnucleusen_US
dc.subjectcytoplasm ratioen_US
dc.subjectsmokeless tobaccoen_US
dc.titleCytological and cytomorphometric characteristics of buccal mucosa cells from smokeless tobacco usersen_US
dc.typeArticleen_US

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