Secukinumab Ameliorates Oxidative Damage Induced by Cerebral Ischemia-Reperfusion in Rats

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dc.authoridCETIN, Asli/0000-0003-3902-3210
dc.contributor.authorOztanir, Mustafa Namik
dc.contributor.authorDogan, Muhammed Fatih
dc.contributor.authorBasak Turkmen, Nese
dc.contributor.authorTaslidere, Asli
dc.contributor.authorSahin, Yasemin
dc.contributor.authorCiftci, Osman
dc.date.accessioned2024-08-04T20:52:19Z
dc.date.available2024-08-04T20:52:19Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAIM: To investigate the histological and biochemical neuroprotective effects of secukinumab (SEC) on cerebral ischemia-reperfusion (IR) injury in Sprague-Dawley male rats. MATERIAL and METHODS: A total of 28 Sprague-Dawley male rats were randomly and equally divided into the following four groups: Sham, SEC, IR, and IR+SEC groups. Bilateral common carotid arteries were simultaneously separated and blocked for 15 minutes using two vascular mini clips in the IR and IR+SEC groups. The surgical procedure was similarly repeated in the Sham and SEC groups, but the carotid arteries were not clipped. Secukinumab was administered intraperitoneally to the SEC and IR+SEC groups once a week after the surgical procedure. Rat brain tissues were collected for biochemical analysis and histopathological examination 14 days after surgery. RESULTS: Cerebral IR caused abnormal changes in oxidative stress parameters by increasing the malondialdehyde (MDA) level and by decreasing the glutathione (GSH), catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD) levels. IR also induced histopathological alterations, such as vascular congestion, hemorrhage, and cell infiltration in the rat brain tissues. Secukinumab treatment significantly decreased the MDA levels and increased the GPx, GSH, CAT, and SOD levels. In addition, secukinumab partially prevented histopathological alterations in the brain tissues. The percentage of immunohistochemically Caspase-3-positive cells was high in the IR group; however, SEC decreased the density of cells stained with Caspase-3. CONCLUSION: IR injury was found to cause oxidative and histopathological changes in rat brain tissues, and secukinumab treatment ameliorated these pathological effects. Therefore, secukinumab may be useful to prevent and treat oxidative stress -induced brain damage in patients with ischemic stroke.en_US
dc.description.sponsorshipInonu University Scientific Research Fund [INU-BAP 2016/180]en_US
dc.description.sponsorshipThis research was supported by Inonu University Scientific Research Fund (INU-BAP 2016/180) .en_US
dc.identifier.doi10.5137/1019-5149.JTN.33606-21.3
dc.identifier.endpage739en_US
dc.identifier.issn1019-5149
dc.identifier.issue5en_US
dc.identifier.pmid35147962en_US
dc.identifier.scopus2-s2.0-85138181918en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage732en_US
dc.identifier.urihttps://doi.org/10.5137/1019-5149.JTN.33606-21.3
dc.identifier.urihttps://hdl.handle.net/11616/100894
dc.identifier.volume32en_US
dc.identifier.wosWOS:000865966600004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTurkish Neurosurgical Socen_US
dc.relation.ispartofTurkish Neurosurgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSecukinumaben_US
dc.subjectNeuroprotectionen_US
dc.subjectCerebral ischemia-reperfusionen_US
dc.subjectOxidative stressen_US
dc.subjectRatsen_US
dc.titleSecukinumab Ameliorates Oxidative Damage Induced by Cerebral Ischemia-Reperfusion in Ratsen_US
dc.typeArticleen_US

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