Comparative study of ASNase immobilization on tannic acid-modified magnetic Fe3O4/SBA-15 nanoparticles to enhance stability and reusability

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dc.authoridAteş, Burhan/0000-0001-6080-229X
dc.authoridUlu, Ahmet/0000-0002-4447-6233
dc.authoridodabaşı, mehmet/0000-0002-3288-132X
dc.authoridSanz-Pérez, Eloy S./0000-0003-1271-0555
dc.authoridAcet, Omur/0000-0003-1864-5694
dc.authoridSanz Martin, Raul/0000-0002-0198-2443
dc.authorwosidAcet, Ömür/IUN-5176-2023
dc.authorwosidAteş, Burhan/AAA-3730-2021
dc.authorwosidAcet, Ömür/ABI-5085-2020
dc.authorwosidUlu, Ahmet/L-5180-2016
dc.authorwosidSanz, Raúl/ABF-3570-2020
dc.authorwosidodabaşı, mehmet/AAQ-8926-2020
dc.authorwosidSanz-Pérez, Eloy S./B-3942-2012
dc.contributor.authorNoma, Samir Abbas Ali
dc.contributor.authorUlu, Ahmet
dc.contributor.authorAcet, Omur
dc.contributor.authorSanz, Raul
dc.contributor.authorSanz-Perez, Eloy S.
dc.contributor.authorOdabasi, Mehmet
dc.contributor.authorAtes, Burhan
dc.date.accessioned2024-08-04T20:47:13Z
dc.date.available2024-08-04T20:47:13Z
dc.date.issued2020
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIn this work, l-asparaginase was immobilized on tannic acid-modified magnetic mesoporous particles. In brief, Fe3O4/SBA-15/tannic acid magnetic particles were synthesized, and their structures and morphologies were fully characterized using various methods. The properties of the free and immobilized enzyme were examined in terms of pH, temperature, thermal stability, storage stability, and reusability. Moreover, the effects of metal ions, inhibitors and organic solvents on the activity of the immobilized enzyme were investigated. Compared to the free enzyme, the immobilized enzyme possessed better tolerance to changes in ambient temperature and pH. Additionally, thermal incubation results showed that the free enzyme lost its activity, while the immobilized enzyme exhibited the opposite behavior. Most strikingly, the immobilized l-asparaginase exhibited a high degree of activity (70%) after being reused 16 times while also demonstrating 71% and 63% storage stability of the initial activity even after 28 days at 4 degrees C and room temperature, respectively. Together with these results, l-asparaginase was successfully immobilized upon Fe3O4/SBA-15/tannic acid magnetic nanoparticles with improved stability properties. This support holds great potential and opens up a novel perspective for growing applications.en_US
dc.identifier.doi10.1039/d0nj00127a
dc.identifier.endpage4451en_US
dc.identifier.issn1144-0546
dc.identifier.issn1369-9261
dc.identifier.issue11en_US
dc.identifier.scopus2-s2.0-85082107211en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage4440en_US
dc.identifier.urihttps://doi.org/10.1039/d0nj00127a
dc.identifier.urihttps://hdl.handle.net/11616/99227
dc.identifier.volume44en_US
dc.identifier.wosWOS:000521102000024en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofNew Journal of Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCore-Shell Nanoparticlesen_US
dc.subjectOne-Pot Synthesisen_US
dc.subjectL-Asparaginaseen_US
dc.subjectRecoverable Catalysten_US
dc.subjectFe3o4 Nanoparticlesen_US
dc.subjectGraphene Oxideen_US
dc.subjectFe3o4-At-Mesoporous Sba-15en_US
dc.subjectMesoporous Silicaen_US
dc.subjectEnzymeen_US
dc.subjectNanocompositeen_US
dc.titleComparative study of ASNase immobilization on tannic acid-modified magnetic Fe3O4/SBA-15 nanoparticles to enhance stability and reusabilityen_US
dc.typeArticleen_US

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