Metal-organic frameworks (MOFs): a novel support platform for ASNase immobilization

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dc.authoridUlu, Ahmet/0000-0002-4447-6233
dc.authorwosidUlu, Ahmet/L-5180-2016
dc.contributor.authorUlu, Ahmet
dc.date.accessioned2024-08-04T20:47:08Z
dc.date.available2024-08-04T20:47:08Z
dc.date.issued2020
dc.departmentİnönü Üniversitesien_US
dc.description.abstractMetal-organic frameworks (MOFs) have been recently studied for a variety of applications because of their huge surface area, large porosity, and tunable functionality. In this work, for the first time, the efficient immobilization of l-asparaginase (ASNase, EC 3.5.1.1) by using MOF as a simple and novel support is demonstrated. The functional groups, morphology, chemical composition, and crystal structure of the support and immobilized ASNase were investigated by using different methods, including Fourier transform infrared spectroscopy, scanning electron microscopy, energy-dispersive spectrometer, and X-ray diffraction. Afterward, the enzymatic activities and thermodynamic parameters of the immobilized l-ASNase (ASNase@ZIF-8) were compared with free one. After enzyme immobilization, the optimum temperature shifted from 50 to 60 degrees C, while the optimum pH remains unchanged at 9.0. However, the pH and thermal stability of the ASNase@ZIF-8 was significantly improved compared to the free one. The ASNase@ZIF-8 displayed an excellent long-term storage stability, which could protect more than 56% of the initial activity at 25 degrees C for 4 weeks. Besides, the ASNase@ZIF-8 had high reusability, which showed a high degree of activity (more than 45%) after 10 cycles. K-m and V-max values were 0.18 mM and 64.5 mu mol/min for ASNase@ZIF-8 and those for free ASNase were 0.40 mM and 68.0 mu mol/min, respectively. The proposed support based on ZIF-8 was superior in terms of high enzyme loading capacity (82.0%), high enzyme catalytic activity, and easy preparation process. Overall, newly developed support for ASNase may provide a new platform for its biotechnological applications.en_US
dc.identifier.doi10.1007/s10853-020-04452-6
dc.identifier.endpage6144en_US
dc.identifier.issn0022-2461
dc.identifier.issn1573-4803
dc.identifier.issue14en_US
dc.identifier.scopus2-s2.0-85079725129en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage6130en_US
dc.identifier.urihttps://doi.org/10.1007/s10853-020-04452-6
dc.identifier.urihttps://hdl.handle.net/11616/99191
dc.identifier.volume55en_US
dc.identifier.wosWOS:000516205400002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Materials Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEnzyme Immobilizationen_US
dc.subjectL-Asparaginaseen_US
dc.subjectLaccaseen_US
dc.subjectEncapsulationen_US
dc.subjectCompositeen_US
dc.subjectDesignen_US
dc.subjectLipaseen_US
dc.titleMetal-organic frameworks (MOFs): a novel support platform for ASNase immobilizationen_US
dc.typeArticleen_US

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