Effectiveness of peptone-yeast extract (P-Y) medium in the cultivation and isolation of Entamoeba histolytica/Entamoeba dispar in Turkish patients

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dc.authoridKURT, ÖZGÜR/0000-0001-5575-588X;
dc.authorwosidKURT, ÖZGÜR/AAG-9543-2019
dc.authorwosidDagci, Hande/B-1840-2008
dc.authorwosidKurt, Özgür/AAB-3100-2020
dc.contributor.authorDagci, H
dc.contributor.authorBalciogju, IC
dc.contributor.authorErtabaklar, H
dc.contributor.authorKurt, O
dc.contributor.authorAtambay, M
dc.date.accessioned2024-08-04T20:13:32Z
dc.date.available2024-08-04T20:13:32Z
dc.date.issued2003
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAmebiasis is a common protozoan infection worldwide, causing serious health problems in both children and adults. Today, almost 10% of the world population is infected with Entamoeba histolytica/Entamoeba dispar. The aims of this study were both the comparison of the reproduction rates,and densities of E. histolytica/E. dispar in Robinson, Dobell-Laidlaw and P-Y culture media and isolation of E. histolytica/E. dispar from stool samples in Peptone-Yeast (P-Y) medium. Trophozoites and cysts of E. histolytica/E. dispar, maintained in Robinson medium, and stool samples of patients with amebiasis were inoculated into P-Y, Robinson and Dobell-Laidlaw culture media. Reproduction rates reached their peak levels 48 h after the inoculation in all culture media. Reproduction rates in P-Y and Robinson media were found similar; however, they were higher than the reproduction rate in Dobell-Laidlaw medium (p < 0.01); there was no statistically significant difference between the reproduction rates of P-Y and Robinson media (p > 0.05). Twelve isolates from 12 patients were cultivated in P-Y medium and checked for reproduction everyday for 7 days. Twelve of the 12 (100%) isolates were cultivated in P-Y medium, indicating that the P-Y was an effective medium for the isolation of E. histolytica/E. dispar in stool samples. According to these results, P-Y medium could be preferred in immunologic, serologic and molecular studies and, thus the definitive diagnosis of amebiasis due to its low cost and simple formula. (C) 2003 Elsevier Science Inc. All rights reserved.en_US
dc.identifier.doi10.1016/S0732-8893(02)00508-4
dc.identifier.endpage130en_US
dc.identifier.issn0732-8893
dc.identifier.issue2en_US
dc.identifier.pmid12614984en_US
dc.identifier.scopus2-s2.0-0345118202en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage127en_US
dc.identifier.urihttps://doi.org/10.1016/S0732-8893(02)00508-4
dc.identifier.urihttps://hdl.handle.net/11616/93689
dc.identifier.volume45en_US
dc.identifier.wosWOS:000181429700006en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofDiagnostic Microbiology and Infectious Diseaseen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectP-Y mediumen_US
dc.subjectRobinsonen_US
dc.subjectDobell-Laidlawen_US
dc.subjectcultureen_US
dc.titleEffectiveness of peptone-yeast extract (P-Y) medium in the cultivation and isolation of Entamoeba histolytica/Entamoeba dispar in Turkish patientsen_US
dc.typeArticleen_US

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