Functionalization of poly-SNS-anchored carboxylic acid with Lys and PAMAM: surface modifications for biomolecule immobilization/stabilization and bio-sensing applications

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dc.authoridTimur, Suna/0000-0002-1981-7577
dc.authoridEMRE, Fatma Bilge/0000-0002-2972-5596
dc.authoridEmre, Fatma Bilge/0000-0002-2972-5596
dc.authoridTanyeli, Cihangir/0000-0003-2270-8635
dc.authoriddemirci uzun, sema/0000-0002-6445-5464
dc.authorwosidTimur, Suna/CAG-0914-2022
dc.authorwosidEMRE, Fatma Bilge/AAS-3969-2020
dc.authorwosidToppare, Levent/ABA-5056-2020
dc.authorwosidEmre, Fatma Bilge/V-5662-2017
dc.authorwosidTanyeli, Cihangir/AAG-4533-2019
dc.authorwosiddemirci uzun, sema/J-9403-2014
dc.contributor.authorDemirci, Sema
dc.contributor.authorEmre, Fatma Bilge
dc.contributor.authorEkiz, Fulya
dc.contributor.authorOguzkaya, Funda
dc.contributor.authorTimur, Suna
dc.contributor.authorTanyeli, Cihangir
dc.contributor.authorToppare, Levent
dc.date.accessioned2024-08-04T20:36:04Z
dc.date.available2024-08-04T20:36:04Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPoly(2-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl) (SNS) acetic acid) was electrochemically deposited on graphite electrodes and functionalized with lysine (Lys) amino acid and poly(amidoamine) derivatives (PAMAM G2 and PAMAM G4) to investigate their matrix properties for biosensor applications. Glucose oxidase (GOx) was immobilized onto the modified surface as the model enzyme. X-Ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) were used to report the surface properties of the matrices in each step of the biosensor construction. The biosensors were characterized in terms of their operational and storage stabilities and the kinetic parameters (K-m(app) and I-max). Three new glucose biosensors revealed good stability, featuring low detection limits (19.0 mu M, 3.47 mu M and 2.93 mu M for lysine-, PAMAM G2- and PAMAM G4-functionalized electrodes, respectively) and prolonged the shelf lives (4, 5, and 6 weeks for Lys-, PAMAM G2- and PAMAM G4-modified electrodes, respectively). The proposed biosensors were tested for glucose detection on real human blood serum samples.en_US
dc.description.sponsorshipTUBA; TUBITAK [110T580]en_US
dc.description.sponsorshipThis study was partially supported by TUBA and TUBITAK 110T580 grants. METU Central Laboratory is acknowledged for XPS analyses and AFM images. Also, METU Health Center is acknowledged for supplying blood serum samples.en_US
dc.identifier.doi10.1039/c2an35472a
dc.identifier.endpage4261en_US
dc.identifier.issn0003-2654
dc.identifier.issn1364-5528
dc.identifier.issue18en_US
dc.identifier.pmid22832474en_US
dc.identifier.scopus2-s2.0-84864986899en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage4254en_US
dc.identifier.urihttps://doi.org/10.1039/c2an35472a
dc.identifier.urihttps://hdl.handle.net/11616/95771
dc.identifier.volume137en_US
dc.identifier.wosWOS:000307450000022en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofAnalysten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlucose-Oxidaseen_US
dc.subjectCarbon Nanotubesen_US
dc.subjectConducting Polymeren_US
dc.subjectHydrogen-Peroxideen_US
dc.subjectBiosensoren_US
dc.subjectDendrimersen_US
dc.subjectMonolayersen_US
dc.subjectBiocatalysten_US
dc.subjectMatrixen_US
dc.subjectFilmen_US
dc.titleFunctionalization of poly-SNS-anchored carboxylic acid with Lys and PAMAM: surface modifications for biomolecule immobilization/stabilization and bio-sensing applicationsen_US
dc.typeArticleen_US

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