Molecular characterization and antibiotic resistance of clinical Bacteroides and related genera from a tertiary care center in Turkiye

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dc.contributor.authorDemir, Mervenur
dc.contributor.authorSoki, Jozsef
dc.contributor.authorTanriverdi, Elif Seren
dc.contributor.authorOzkul, Ceren
dc.contributor.authorMahmood, Bakhtiyar
dc.contributor.authorOtlu, Baris
dc.contributor.authorHazirolan, Gulsen
dc.date.accessioned2026-04-04T13:35:13Z
dc.date.available2026-04-04T13:35:13Z
dc.date.issued2024
dc.departmentİnönü Üniversitesi
dc.description.abstractObjectives: This study was conducted to measure the prevalence of antibiotic resistance, and corresponding resistance genes among Bacteroides and related genera in a tertiary hospital. Methods: We examined 138 clinical strains of Bacteroides, Phocaeicola and Parabacteroides species isolated between July 2018 and June 2022. Antibiotic susceptibility tests were conducted using agar dilution. The bft gene and antibiotic resistance genes were targeted by real-time PCR. Results: Resistance rates of all strains against ampicillin, cefoxitin, piperacillin-tazobactam, meropenem, imipenem, clindamycin, metronidazole, and tigecycline were 97.8 %, 28.3 %, 11.6 %, 7.9 %, 5.1 %, 47.8 %, 0 % and 4.3 %, respectively. Non-fragilis Bacteroidales spp. (NFB) exhibited lower susceptibility rates compared to B. fragilis for cefoxitin, clindamycin, and piperacillin-tazobactam. The prevalence of meropenem resistance was higher in B. fragilis (15.5 %) than in NFB (0 %). Among all strains, the rates of cepA, cfxA, cfiA, ermF, ermG, ermB, nim, linA, mefA, msrSA, tetQ, tetX, tetX1 and bft genes were 42.8 %, 44.9 %, 8.7 %, 44.2 %, 10.9 %, 2.2 %, 0.7 %, 29.0 %, 17.4 %, 7.2 %, 76.1 %, 8.0 %, 37.7 % and 16.7 %, respectively. In five B. fragilis strains, insertion sequences [IS1187(n = 3), ISBf6(n = 1), IS612B(n = 1)] were detected in the upstream region of cfiA. NimE with ISBf6 on plasmid pBFM29b was detected in one B. fragilis strain, intermediate to metronidazole (MIC = 16 mu g/mL). ErmF was the most abundant gene responsible for clindamycin resistance. TetQ and tetX1 genes exhibited a higher frequency in strains that were not susceptible to tigecycline (MIC >= 8 mu g/ml). Conclusions: Monitoring the resistance trends of Bacteroides and related genera is crucial given the observed resistance to all classes of antibiotics and the presence of various resistance mechanisms.
dc.description.sponsorshipHacettepe University Scientific Research Project Unit [THD-2021-19036]
dc.description.sponsorshipThis study was supported by the Hacettepe University Scientific Research Project Unit (Project No: THD-2021-19036) .
dc.identifier.doi10.1016/j.anaerobe.2024.102912
dc.identifier.issn1075-9964
dc.identifier.issn1095-8274
dc.identifier.orcid0000-0002-0449-0356
dc.identifier.orcid0000-0002-0921-5863
dc.identifier.orcid0000-0001-9229-0874
dc.identifier.pmid39326493
dc.identifier.scopus2-s2.0-85204979138
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.anaerobe.2024.102912
dc.identifier.urihttps://hdl.handle.net/11616/109701
dc.identifier.volume90
dc.identifier.wosWOS:001327983600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofAnaerobe
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250329
dc.subjectBacteroides fragilis
dc.subjectAntibiotic resistance
dc.subjectResistance gene
dc.subjectbft gene
dc.subjectInsertion sequence
dc.subjectCarbapenem
dc.titleMolecular characterization and antibiotic resistance of clinical Bacteroides and related genera from a tertiary care center in Turkiye
dc.typeArticle

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