Detection and typing of extended-spectrum ?-lactamases in clinical isolates of the family Enterobacteriaceae in a medical center in Turkey

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dc.authoridTekerekoglu, Mehmet Sait/0000-0001-7284-3427
dc.authoridkoroglu, mehmet/0000-0001-8101-1104
dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authoridDURMAZ, RIZA/0000-0001-6561-778X
dc.authorwosidTekerekoglu, Mehmet Sait/ABI-8148-2020
dc.authorwosidDurmaz, Berrin/AAA-2213-2021
dc.authorwosidkoroglu, mehmet/HTN-0171-2023
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.contributor.authorDurmaz, R
dc.contributor.authorDurmaz, B
dc.contributor.authorKoroglu, M
dc.contributor.authorTekerekoglu, MS
dc.date.accessioned2024-08-04T20:12:16Z
dc.date.available2024-08-04T20:12:16Z
dc.date.issued2001
dc.departmentİnönü Üniversitesien_US
dc.description.abstractTo determine and type the extended-spectrum beta -lactamases (ESBLs) among the family Enterobacteriaceae in a medical center, a total of 668 clinical isolates were screened. Of the 668 isolates, the 80 strains were presumptively defined as ESBL producers according to the result of disk method using ESBL marker antibiotics (aztreonam, ceftazidime, and cefoxitin). These 80 strains were retested with the double-disk synergy test (DDST), the E-test ESBL strip, a 5-mug ceftazidime disk, and agar dilution MICs of ceftazidime with and without clavulonic acid. Isoelectric focusing was performed to confirm ESBL production and type the beta -lactamases. By evaluation of the results of all tests used for ESBL detection together with isoelectric focusing, 33 (4.9%) of the 668 isolates were described as ESBL producer. The positive results of the agar dilution test, DDST, the E-test strip, and 5-mug ceftazidime disk were 32, 26, 27, and 26 of the 33 strains, respectively. ESBL positivity was 48.8% in Klebsiella species, 15.4% in Citrobacter species, 4.9% in Enterobacter species and 1.1% in Escherichia coli strains. The ESBL enzymes frequently determined were SHV-2/6-like (pI 7.6), SHV-5-like (pI 8.2), SHV-4-like (pI 7.8), and SHV-3-like (pI 7). SHV-derived enzymes were commonly observed in Klebsiella spp whereas TEM-related enzymes were seen in E. coli strains. The results of this study indicated that SHV-2/6-derived (pI 7.6) ESBL expression among the isolates of the family Enterobacteriaceae is an important problem in our medical center.en_US
dc.identifier.doi10.1089/10766290152045048
dc.identifier.endpage175en_US
dc.identifier.issn1076-6294
dc.identifier.issn1931-8448
dc.identifier.issue2en_US
dc.identifier.pmid11442343en_US
dc.identifier.scopus2-s2.0-0034959690en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage171en_US
dc.identifier.urihttps://doi.org/10.1089/10766290152045048
dc.identifier.urihttps://hdl.handle.net/11616/93299
dc.identifier.volume7en_US
dc.identifier.wosWOS:000169458100006en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofMicrobial Drug Resistanceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectKlebsiella-Pneumoniae Resistanten_US
dc.subjectEscherichia-Colien_US
dc.subjectPrevalenceen_US
dc.subjectStrainsen_US
dc.subjectEsblen_US
dc.subjectEpidemiologyen_US
dc.subjectHospitalsen_US
dc.subjectOutbreaken_US
dc.subjectMembersen_US
dc.titleDetection and typing of extended-spectrum ?-lactamases in clinical isolates of the family Enterobacteriaceae in a medical center in Turkeyen_US
dc.typeArticleen_US

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