F2 nesil farelerde indol-3-asetik asitin böbrek katalaz, süperoksit dismutaz ve glutatyon peroksidaz aktiviteleri üzerine olan etkisi
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Dosyalar
Tarih
2004
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Van Tıp Dergisi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Öz: Amaç: İndol-3-asetik asit (lAA)'in, reaktif oksijen türlerini (ROT) oluşturduğu bilinmektedir. IAA böbrek yetmezliği, hipoglisemi, myotoniye sebep olmaktadır. Bu çalışmada, İAA'in F2 nesil farelerde böbrek katalaz (CAT), süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GSH-Px) enzim aktiviteîerine etkisi araştırıldı. Metod: Dişi fareler (Mus musculus) üç gruba ayrıldı. İki dişi bir erkekle çiftleştirildi. Birinci gruptaki dişilere, İAA'in 300mg/kg vücut ağırlığı dozu etanolde 1/40 seyreltilerek üç günde bir deri altına verildi. İkinci gruptaki dişilere, kontrol grubu olarak etanol aynı yolla verildi. Üçüncü gruptakilere de, kontrol etanolun miktarı kadar serum fizyolojik verildi. Bu işlemler iki nesil boyunca yapıldı. Böbrek dokusunda enzim aktiviteleri spekrofotornetrik olarak tayin edildi. Bulgular: IAA verilen gruptaki F2 nesilde, CAT aktivitesi kontrol etanol grubuna göre istatistiksel olarak anlamlı bir şekilde azaldı (p<0.002). Gruplar birbirleriyle karşılaştırıldığında SOD ve GSH-Px aktivitelerinde anlamlı bir fark gözlenmedi.
Abstract: Aim: Indole-3-acetic acid (IAA) has been known to generate reactive oxygen species (ROS). IAA causes renal dysfunction, hypoglycemia, and myotonia. In this study, the effect of ındule-3-acelic acid on mice catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities in the kidneys the second generation were investigated. Methods: Female mice (Mus musculus) were divided into 3 groups. For mating, two females and one male were held in a steinless steel cage. IAA was intraperitoneally administrated to maternal mice as 1/40 dilution of 300 mg/kg body weight in 3 day intervals. As controls, ethanol and serum physiologic were administrated. This experimental treatment was carried out for over 2 generations. Spectrophotometric methods were used to determine the activities of above-mentioned enzymes in the kidney tissue. Results: In the second generation of IAA administrated mice, CAT activity was found to be lower than control ethanol group (p<0.002). There was no significant difference in the SOD and GSH-Px activities among IAA, control ethanol, and control serum physiologic groups. Conclusion: As a result, we can say that the CAT was affected negatively from IAA toxicity.
Abstract: Aim: Indole-3-acetic acid (IAA) has been known to generate reactive oxygen species (ROS). IAA causes renal dysfunction, hypoglycemia, and myotonia. In this study, the effect of ındule-3-acelic acid on mice catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities in the kidneys the second generation were investigated. Methods: Female mice (Mus musculus) were divided into 3 groups. For mating, two females and one male were held in a steinless steel cage. IAA was intraperitoneally administrated to maternal mice as 1/40 dilution of 300 mg/kg body weight in 3 day intervals. As controls, ethanol and serum physiologic were administrated. This experimental treatment was carried out for over 2 generations. Spectrophotometric methods were used to determine the activities of above-mentioned enzymes in the kidney tissue. Results: In the second generation of IAA administrated mice, CAT activity was found to be lower than control ethanol group (p<0.002). There was no significant difference in the SOD and GSH-Px activities among IAA, control ethanol, and control serum physiologic groups. Conclusion: As a result, we can say that the CAT was affected negatively from IAA toxicity.
Açıklama
Van Tıp Dergisi
Yıl: 2004Cilt: 11Sayı: 3ISSN: 1300-2694 / 2587-0351Sayfa Aralığı: 64 - 68Metin Dili:Türkçe
Anahtar Kelimeler
katalaz
Kaynak
Van Tıp Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
11
Sayı
3
Künye
YILMAZ H. R,YÜKSEL E,TÜRKÖZ Y (2004). F2 nesil farelerde indol-3-asetik asitin böbrek katalaz, süperoksit dismutaz ve glutatyon peroksidaz aktiviteleri üzerine olan etkisi. Van Tıp Dergisi, 11(3), 64 - 68.
