Comparative biological evaluation and identification of multifunctional endogenous and exogenous bioactive peptides in different matured Izmir Tulum cheeses

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dc.contributor.authorAkan, Ecem
dc.contributor.authorTok, Kerem
dc.contributor.authorKocazorbaz, Ebru Kocadag
dc.contributor.authorZihnioglu, Figen
dc.contributor.authorYerlikaya, Oktay
dc.contributor.authorHayaloglu, Ali Adnan
dc.contributor.authorKinik, Ozer
dc.date.accessioned2026-04-04T13:35:04Z
dc.date.available2026-04-04T13:35:04Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractBioactive peptides redefine health and disease by triggering unique biological functions in the body. The aim of this study was to evaluate and identify the endogenous and exogenous peptides (in vitro gastrointestinal digestion) of 360-day ripened Izmir Tulum cheese manufactured using cow, sheep, and goat milk. Peptide extracts from Izmir Tulum cheeses were found to have DPP-IV inhibitory, deglycation, antioxidant, metal chelating, and ACE-inhibitory activity. After the activity guided purification of peptide extracts were performed by HPLC, 8 peptide fractions were selected according to their biological activity, and the peptide sequence of each peptide were identified by mass spectrometry. It was observed that the use of different types of milk in the manufacture of Izmir Tulum cheese affects peptide diversity, that endogenous and exogenous peptides had a wide range of potential biological activities, and that new bioactive peptides were obtained from Izmir Tulum cheese. The potential peptide sequences obtained from the identified fractions ETFHPLKHQ, EVVPGPFPQ, and TLGLHTPK are the first peptide sequences that do not overlap with sequences published in the literature. In conclusion, these endogenous and exogenous peptides of Izmir Tulum cheeses have significant biological activities associated with type 2 diabetes, deglycation, antioxidants, and metal chelating agents to support the immune system, and ACEinhibitory effects to treat hypertension. These new peptides could open a new avenue for the noval peptide-based drug and contribute to therapeutic applications.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [118R022]; TUBITAK; Aliye Uster Foundation
dc.description.sponsorshipThis study was supported by Scientific and Technological Research Council of Turkey (TUBITAK) under the Grant Number 118R022. The authors thank to TUBITAK for their supports. Additionaly, authors thank to Aliye Uster Foundation for their financial support.
dc.identifier.doi10.1016/j.idairyj.2024.106141
dc.identifier.issn0958-6946
dc.identifier.issn1879-0143
dc.identifier.orcid0000-0002-1532-4687
dc.identifier.orcid0000-0002-4274-2729
dc.identifier.scopus2-s2.0-85209363791
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.idairyj.2024.106141
dc.identifier.urihttps://hdl.handle.net/11616/109599
dc.identifier.volume161
dc.identifier.wosWOS:001363972400001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofInternational Dairy Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectDairy products
dc.subjectIzmir Tulum cheese
dc.subjectIn vitro digestion
dc.subjectAntidiabetic activity
dc.subjectDeglycation
dc.subjectAntihypertensive activity
dc.titleComparative biological evaluation and identification of multifunctional endogenous and exogenous bioactive peptides in different matured Izmir Tulum cheeses
dc.typeArticle

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