The effect of Nateglinide and Octreotide on follicular morphology and free radical scavenging system in letrazole-induced rat model of PCOS
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Verduci Publisher
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
OBJECTIVE: To investigate the ef-fects of octreotide and nateglinide on ovarian folli-cle count, ovarian tissue damage, biochemical pa-rameters and free radical scavenging system in le-trazole-induced rat model of PCOS. MATERIALS AND METHODS: Forty-two female Sprague-Dawley rats were divided into six groups. Group 1 (Control Group): after localizing the ova-ries and the uterine horns, the abdominal wall was closed without any surgical procedure. Group 2 (PCOS Group): PCOS was induced by administrat-ing Letrozole orally for 21 successive days. At the end of 21 days, rats underwent ovarian biopsies. The experimental PCOS model was considered successful in the presence of atretic follicles with-out granulosa cell stratification. Group 3 (PCOS + Nateglinide Group): Nateglinide was administered by oral dropper for 30 days to the rats in which PCOS model was created. Group 4 (Nateglinid only Group): 30 days of NG was applied to the rats with-out PCOS. Group 5 (PCOS+Octreotide Group): 0.1 mg/kg/day Octreotide was given intraperitoneally for 4 weeks to the rats in which PCOS model was created. Group 6 (Octreotide only Group): animals without PCOS were given 0.1 mg/kg/day Octreotide. Bilateral oophorectomy was performed and blood samples were collected from all groups at the end of the treatment. Ovarian tissue was stained immu-nohistochemically with TLR-4 in addition to con-ventional staining. In addition to follicle classifica-tion, ovarian damage was graded. Serum insulin, FSH and LH, TNF-alpha, IL-6, SHBG, SOD, IGF-1, MDA and GSH levels were also measured. RESULTS: The cystic and degenerated follicle density of PCOS group was high compared with the other groups. Both cystic and degenerated fol- licles were significantly reduced in PCOS+NG and PCOS+OC groups compared to PCOS group. There was no difference between the groups in terms of serum LH, FSH and insulin levels (p > 0.05). Serum testosterone level was significantly higher in the PCOS group compared to the other groups (p < 0.01). Adding OC or NG to PCOS groups did not cause significant changes in testosterone levels. TNF-alpha and IL-6 levels were high in PCOS group (p < 0.03). IGF-1 and MDA levels were higher in PCOS than in other groups (p < 0.03, p < 0.01 respectively). Adding OC or NG to the treatment normalized IGF-1 and MDA levels. Serum GSH levels were significantly lower in the PCOS group (p < 0.05). Adding NG to the treatment increased GSH levels. CONCLUSIONS: Both NG and OCT reverses atretic and degenerate follicle damage due to PCOS through TLR-4, antioxidant and anti-inflammatory pathways.
Açıklama
Anahtar Kelimeler
Key PCOS, Letrazol, NG, OCT, Follicle, Oxidation, In-flammation
Kaynak
European Review For Medical and Pharmacological Sciences
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
26
Sayı
23
