Identification of biodiversity of some Streptomyces species and determination of a restriction fragment length polymorphism (RFLP) profile of 16S rDNA gene region

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dc.authorscopusid25230283000
dc.authorscopusid22986499400
dc.authorscopusid56385361600
dc.authorscopusid56343258200
dc.authorscopusid6602206186
dc.contributor.authorOzdemir K.
dc.contributor.authorOgun E.
dc.contributor.authorErtas M.
dc.contributor.authorAcar S.
dc.contributor.authorAtalan E.
dc.date.accessioned2024-08-04T20:02:33Z
dc.date.available2024-08-04T20:02:33Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIn this study, soil samples from the roots and peripheral regions of some agricultural plant species growing in the Van region (such as sainfoin, bean, malus, wheat and sugar beet) were collected between May and June 2004 and 139 strains of Streptomyces bacteria were isolated from these samples. Moreover, researchers determined the physicochemical characteristics of these soil samples. Streptomyces bacteria were color-grouped and the Diaminopimelic Acid (DAP) forms were detected followed by the determination of then phenotypic characteristics. When these isolates were divided into 39 color groups, 20 strains were found to contain L-DAP and therefore were confirmed as members of the genus Streptomyces. The species were identified using the TAXON Software and similarity dendrograms were constructed by the Unweighted Pair-Group Method with an Arithmetic average (UPGMA) cluster analysis using the Multi-Variate Statistical Package (MVSP 3.1). Genomic DNA was isolated from these bacteria and 16S rDNA gene was amplified by PCPv followed by restriction digestion with Bspl43I, Haelll, Mnll and SphI for Restriction Fragment Length Polymorphism (RFLP) analysis. In the dendrogram obtained at the end of the RFLP analysis, four groups of two or more members and six 1 -member groups were established according to the similarity ratio criterion. © Medwell Journals, 2014.en_US
dc.identifier.doi10.3923/javaa.2014.978.988
dc.identifier.endpage988en_US
dc.identifier.issn1680-5593
dc.identifier.issue16en_US
dc.identifier.scopus2-s2.0-84907988031en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage978en_US
dc.identifier.urihttps://doi.org/10.3923/javaa.2014.978.988
dc.identifier.urihttps://hdl.handle.net/11616/91768
dc.identifier.volume13en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMedwell Journalsen_US
dc.relation.ispartofJournal of Animal and Veterinary Advancesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject16S rDNAen_US
dc.subjectIsolationen_US
dc.subjectRatio criterionen_US
dc.subjectRFLPen_US
dc.subjectStreptomycesen_US
dc.titleIdentification of biodiversity of some Streptomyces species and determination of a restriction fragment length polymorphism (RFLP) profile of 16S rDNA gene regionen_US
dc.typeArticleen_US

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