Ischemia-reperfusion leads to depletion of glutathione content and augmentation of malondialdehyde production in the rat heart from overproduction of oxidants: Can caffeic acid phenethyl ester (CAPE) protect the heart?

Basit Öğe Kaydı
dc.authoridVardı, Nigar/0000-0003-0576-1696
dc.authoridCigremis, Yilmaz/0000-0002-8600-0946
dc.authoridAcet, Ahmet/0000-0003-1131-1878
dc.authoridParlakpinar, Hakan/0000-0001-9497-3468
dc.authoridParlakpınar, Hakan/0000-0001-9497-3468
dc.authorwosidCigremis, Yilmaz/JAC-8451-2023
dc.authorwosidVardı, Nigar/C-9549-2018
dc.authorwosidCigremis, Yilmaz/O-6019-2015
dc.authorwosidAcet, Ahmet/AAB-3273-2021
dc.authorwosidParlakpinar, Hakan/V-6637-2019
dc.authorwosidParlakpınar, Hakan/T-6517-2018
dc.contributor.authorOzer, MK
dc.contributor.authorParlakpinar, H
dc.contributor.authorCigremis, Y
dc.contributor.authorUcar, M
dc.contributor.authorVardi, N
dc.contributor.authorAcet, A
dc.date.accessioned2024-08-04T20:14:46Z
dc.date.available2024-08-04T20:14:46Z
dc.date.issued2005
dc.departmentİnönü Üniversitesien_US
dc.description.abstractDuring restoration of blood flow of the ischemic heart induced by coronary occlusion, free radicals cause lipid peroxidation with myocardial injury. Lipid peroxidation end-products, such as malondialdehyde (MDA), have been used to assess oxygen free radical-mediated injury of the ischemic-reperfused (I/R) myocardium in rats. This experimental study assessed the preventive effect of caffeic acid phenthyl ester (CAPE), antioxidant, on I/R-induced lipid peroxidation in the rat heart. We are also interested in the role of CAPE on glutathione (GSH) levels, an antioxidant whose levels are influenced by oxidative stress. I/R leads to the depletion of GSH which is the major intracellular nonprotein sulphydryl and plays an important role in the maintenance of cellular proteins and lipid in their functional state and acts primarily to protect these important structures against the threat of oxidation. In addition, we also examined morphologic changes in the heart by using light microscopy. The left coronary artery was occluded for 30 min and then reperfused for 120 min more before the experiment was terminated. CAPE (50 mu M kg(-1)) was administered 10 min prior to ischemia and during occlusion by infusion. At the end of the reperfusion period, rats were sacrificed, and the heart was quickly removed for biochemical determination and histopathological analysis. I/R was accompanied by a significant increase in MDA production and decrease in GSH content in the rat heart. Administration of CAPE reduced MDA production and prevented depletion of GSH content. These beneficial changes in these biochemical parameters were also associated with parallel changes in histopathological appearance. These findings imply that I/R plays a causal role in heart injury due to overproduction of oxygen radicals or insufficient antioxidant and CAPE exert cardioprotective effects probably by the radical scavenging and antioxidant activities.en_US
dc.identifier.doi10.1007/s11010-005-0551-8
dc.identifier.endpage175en_US
dc.identifier.issn0300-8177
dc.identifier.issn1573-4919
dc.identifier.issue1-2en_US
dc.identifier.pmid16013452en_US
dc.identifier.scopus2-s2.0-20044387961en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage169en_US
dc.identifier.urihttps://doi.org/10.1007/s11010-005-0551-8
dc.identifier.urihttps://hdl.handle.net/11616/93959
dc.identifier.volume273en_US
dc.identifier.wosWOS:000229219800018en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular and Cellular Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcaffeic acid phenethyl ester (CAPE)en_US
dc.subjectischemia-reperfusionen_US
dc.subjectmalondialdehyde (MDA)en_US
dc.subjectglutathione (GSH)en_US
dc.subjecthearten_US
dc.subjectraten_US
dc.titleIschemia-reperfusion leads to depletion of glutathione content and augmentation of malondialdehyde production in the rat heart from overproduction of oxidants: Can caffeic acid phenethyl ester (CAPE) protect the heart?en_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu