Ameliorative effects of aminoguanidine on rat aorta in Streptozotocin induced diabetes and evaluation of SMA expression

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dc.authoridTR131655en_US
dc.authoridTR45132en_US
dc.authoridTR131654en_US
dc.authoridTR58662en_US
dc.contributor.authorElbe, Hülya
dc.contributor.authorVardı, Nigar
dc.contributor.authorOrman, Doğan
dc.contributor.authorTaşlıdere, Elif
dc.contributor.authorYıldız, Azibe
dc.date.accessioned2017-04-11T11:22:42Z
dc.date.available2017-04-11T11:22:42Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjective: Diabetes mellitus is one of the chronic metabolic diseases which is characterized by microvascular and macrovascular complications. This study was designed to investigate the improving the effects of amnioguanidine on aortic damage in a streptozotocin (STZ) induced diabetic rat model. Methods: Thirty-two male Sprague-Dawley rats divided into four groups as follows: Control, Aminoguanidine, Diabetes, and Diabetes+Aminoguanidine. Experimental diabetes was induced by single dose STZ (45 mg/kg) intraperitoneally. After administration of STZ, the DM+AMG group began to receive AMG (1 g/L) was prepared by dissolving in tap water during 10 weeks. At the end of the study, blood glucose levels were determined and rats were sacrified by ketamine anesthesia. Following routine tissue process, aortas were embedded in paraffin. Histochemical (H-E and Orcein) and immunohistochemical α-smooth muscle actin (α-SMA) stains were applied and the sections examined by light microscope. Statistical analysis was carried out using the SPSS 13.0 statistical program. Results: The rats in diabetes group had significantly higher blood glucose levels than the rats of control. The main histological alterations were detected in tunica media such as extensive thickening (414.32±9.62 μm), irregular of elastic fibers and intensive α-SMA staining in diabetic rats. The thickness of tunica media was statistically increased in DM group, when compared with the control group (p<0.001). On the other hand, AMG prevented disorganization of elastic fibers and overexpression of α-SMA. The mean thickness of tunica media was decreased significantly in DM+AMG (319.16±6.53 μm) compared with the DM group (p<0.001). Conclusion: Our results demonstrate that AMG treatment may protect the impairment of aort structure at histological level.en_US
dc.identifier.citationElbe, H. Vardı, N. Orman, D. Taşlıdere, E. Yıldız, A. (2014). Ameliorative effects of aminoguanidine on rat aorta in Streptozotocin induced diabetes and evaluation of SMA expression. Anadolu Kardiyoloji Dergisi/The Anatolian Journal of Cardiology, 14(8), 679–684.en_US
dc.identifier.doi10.5152/akd.2014.5047en_US
dc.identifier.endpage684en_US
dc.identifier.issn13028723
dc.identifier.issue8en_US
dc.identifier.startpage679en_US
dc.identifier.urihttp://anatoljcardiol.com/jvi.aspx?un=AJC-34445
dc.identifier.urihttps://hdl.handle.net/11616/6636
dc.identifier.volume14en_US
dc.language.isoengen_US
dc.publisherAnadolu Kardiyoloji Dergisi/The Anatolian Journal of Cardiologyen_US
dc.relation.ispartofAnadolu Kardiyoloji Dergisi/The Anatolian Journal of Cardiologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDiabetesen_US
dc.subjectAminoguanidineen_US
dc.subjectA-smooth muscle actinen_US
dc.subjectNitric oxideen_US
dc.subjectAortaen_US
dc.subjectRaten_US
dc.titleAmeliorative effects of aminoguanidine on rat aorta in Streptozotocin induced diabetes and evaluation of SMA expressionen_US
dc.typeLetteren_US

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